Beckman Coulter Application (system and method support)
Automation of Genomic DNA from Cell Cultures and Mouse Tails on the Biomek® 2000 Laboratory Automation Workstation using Promega's Wizard* SV 96 Genomic DNA Purification System
Matthew Cu**, Dana P. Campbell**, Keith Roby**, and Hobert Wai**
Beckman Coulter, Inc.
**Indianapolis, IN and ***Fullerton, CA
Terri Grunst and Daniel Kephart
Promega Corporation –
www.promega.com
We have developed multiple automated methods for the isolation of genomic DNA from tissue cell cultures and mouse tail clippings on the Biomek 2000 to avoid a labor-intensive and time-consuming procedure that has been susceptible to contamination from different sources. Specifically, we have developed multiple automated methods for the isolation of genomic DNA using the Promega Wizard SV 96 Genomic Purification System (Promega, Inc., Madison, WI) and the Biomek® 2000 Laboratory Automation Workstation from Beckman Coulter. By using a 96-well vacuum filtration procedure to isolate and purify genomic DNA from cell and mouse-tail lysates, we have eliminated the need for centrifugation. Washing the bound genomic DNA requires no disassembly of the vacuum manifold and filtrate waste products are delivered directly to a vacuum trap. Genomic DNA is eluted into a convenient 96-well deep-well plate for convenient downstream sample analysis, making the process simple and easy to implement in the genomic research laboratory. The purified genomic DNA has been evaluated for quality, reproducibility, degradation, and cross-contamination using spectrophotometry, agarose gel electrophoresis, and PCR* analysis.
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.
