eLabNotebook - GeneChip Expression Analysis on Biomek® 3000 ArrayPlex

eLabNotebook > Nucleic Acid Prep & Purification > Target Preparation > GeneChip Expression
Analysis on Biomek® 3000 ArrayPlex

Target Preparation for GeneChip* Expression Analysis Using the Biomek® 3000 Laboratory Automation Workstation and ArrayPLEX Application

Zhu Zhu, Dan Allison, Yu Suen, Handy Yowanto, Tim Lancaster, Jeremy Smith and Keith Roby,
Beckman Coulter, Inc., Fullerton, California.

Biomek® 3000 system configuration:

Biomek® Software version 3.3.14
Biomek® 3000 ArrayPLEX System
Please Contact your local sales representative for Biomek® 3000 ArrayPLEX Methods.

Biomek® 3000 ArrayPLEX System Options

Source

Part No.

Quantity

Description

Beckman Coulter, Inc.

A34877

1

Biomek® 3000 ArrayPLEX System Upgrade with Thermal Cycler Integration

Beckman Coulter, Inc.

A34878

1

Biomek® 3000 ArrayPLEX System Upgrade without Thermal Cycler Integration

Beckman Coulter, Inc.

A34875

1

Biomek® 3000 ArrayPLEX System with Thermal Cycler Integration

Beckman Coulter, Inc.

A34876

1

Biomek® 3000 ArrayPLEX System without Thermal Cycler Integration

Materials

cDNA Synthesis

Source	Part No.	Quantity	Description
Ambion	AM7852	1	Cervical Adenocarcinoma (HeLa-S3) Total RNA*,1mg/ml
Affymetrix	900433	1	GeneChip* Eukaryotic Poly-A RNA Control Kit
Affymetrix	900431	1	GeneChip Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis kit

IVT

Source	Part No.	Quantity	Description
Ambion	AM2085	1	T7 RNA Polymerase (Cloned)* 200 U/µl
Affymetrix	900449	1	GeneChip Expression 3’-Amplification Reagents for IVT Labeling

cDNA/cRNA Cleanup

Source	Part No.	Quantity	Description
Agencourt	600560	1	Agencourt® RNACleanTM 96 Starter Kit
Sigma Aldrich	E7023	1	Absolute Ethanol-500 ML*

GeneChip Hybridization

Source	Part No.	Quantity	Description
Affymetrix	900467	1	GeneChip Human Genome U133 Plus 2.0 Array
Affymetrix	900721	1	GeneChip Wash Buffers
Affymetrix	900720	1	GeneChip Hybridization, Wash, and Stain Kit

Others

Source	Part No.	Quantity	Description
Agilent	5067-1511	1	RNA 6000 Nano Chip Kit*
Ambion	9922	1	DEPC treated water*
Gift from DNA & Protein MicroArray Facility at the University of California, Irvine	N/A	1	5X Fragmentation Buffer

* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.