eLabNotebook > Protein Research > Proteomic Applications Biomek® NX
Beckman Coulter Application (system support with verified methods)
Automated Proteomic Applications on Beckman Coulter’s Biomek® NX Laboratory Automation Workstation
Laura Pajak**, Chad Pittman**, Ruth Zhang**, and Scott Boyer**
Beckman Coulter, Inc.
**Indianapolis, IN and ***Fullerton, CA
The information included in this poster describes the utilization of a new automated liquid handler, the Biomek NX Laboratory Automation Workstation, for proteomic applications. His-Tag proteins were purified from bacterial cultures in 96-well plates. Following purification, aliquots from the purified samples were quantitated on the Biomek NX using a Bradford assay. An integrated gripper on the span pod of the Biomek NX permits the microtiter plate containing the quantitation samples to be placed into an integrated reader, the AD 340 Automated Labware Positioner (ALP). This allows for complete walk-away automation of protein purification and quantitation.
The following system components for the proteomic applications will be described:
• The hardware requirements for the Biomek NX
• Software and methods to drive the automation workstation.
Results obtained when purifying proteins, from both uninduced and induced bacterial cultures, on the worksurface of the Biomek NX will be described.
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