Beckman Coulter Application (system support with verified methods)
Automation of Caco-2 Cell Preparation, Drug Permeation and Transport Assay on the Biomek® 3000 Laboratory Automation Workstation
Yu Suen**, Keith Roby**, Javorka Gunic**, Michael Simonian**, and
Graham Threadgill**.
Beckman Coulter, Inc.
**Indianapolis, IN and ***Fullerton, CA
Using Beckman Coulter’s CellProbe HT Caspase-3/7 Whole Cell Assay Kit in primary or secondary screening provides quality information on cellular responses to apoptotic regulators. This 35 µL total volume homogenous assay measures caspase-3/7 activities in a 384-well format. The high-throughput and low sample volume cell-based assay is achieved using the Biomek 3000 Laboratory Automation Workstation for cell preparation, apoptosis induction and substrate addition.
The Affinity* Multi-Mode Plate Reader (Cambridge Research Institute, Boston) is an ultra-sensitive screening platform that uses a focused laser and CCD camera for fluorescence intensity detection. The caspase-3/7 activity is inducerdose dependent and responder-cell number dependent. The dose response of DEVD-CHO inhibition of caspase-3/7 activities was used to demonstrate the superior sensitivity and specificity of this system.
The Biomek 3000 Laboratory Automation Workstation facilitated CellProbe HT Caspase-3/7 Whole Cell Assay implementation, reduced the chance of contamination and minimized the intensive requirement of sterile skills. The automated CellProbe assay can be used for high-throughput screening of drug candidates for apoptosis regulators.
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