Using the Biomek® 3000 Laboratory Automation Workstation to Interface 2D Liquid Chromatography with Mass Spectrometry for Multidimensional Proteome Profiling
Michael Simonian**, Matthew Cu**, Edna Betgovargez**, Keith Roby**
and Graham Threadgill**
Beckman Coulter, Inc.
**Indianapolis, IN and ***Fullerton, CA
Note: These methods have been verified to work by the Beckman Coulter Applications Team.
They are provided to you as a recommended template to start to develop methods that meet
your particular needs.
Biomek Software v3.x or higher.
Biomek® 3000 method: B3K MALDI Spotting.zip (145 KB)
To install the Multidimensional Proteome Profiling methods on the Biomek® 3000:
- Download the self-extracting archive containing the methods and import files.
- Double-click the file to extract.
- Choose a directory to copy the files.
- Open the Biomek Software program.
- To import the method, go to the File menu and choose Import.
- Navigate to the directory where the extract files are located.
- Select the appropriate file that appears in the Import Method Window and click Open.
If labware and deck definition are needed follow the additional steps below:
- To import labware:
- To import the labware associated with that method go to the Project menu and select Import Project.
- Navigate to the directory where the extracted files are located.
- Select the appropriate file that appears in the Import Project Window and click Open.
- To import the deck:
- To import the deck associated with that method, go to the Instrument menu and select Import/Export Utility.
- Click Open.
- Navigate to the directory where the extracted files are located.
- Select the appropriate file that appears in the Open Import
File Window and click Open.
Note: This will be the same file that was used to import the labware into the project folder. - In the Import File Window select the deck to be imported.
- Click Import to import the deck.
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