Automation of Amplification and Primer Extension Chemistries for Beckman Coulter’s GenomeLab™ SNPstream® Genotyping System
Keith Roby**, Laura Pajak**, Dana Campbell**, Zhiming Jiang**, Chad
Pittman** and Scott Boyer.
Beckman Coulter, Inc.
**Indianapolis, IN and ***Fullerton, CA
Note: These methods have been verified to work by the Beckman Coulter Applications Team.
They are provided to you as a recommended template to start to develop methods that meet
your particular needs.
Biomek Software v3.x or higher.
Pre-PCR SNPstream methods: Pre-PCR.zip (24 KB)
Post-PCR SNPstream methods: Post-PCR.zip (113 KB)
To install the SNPstream methods on the GenomeLab™ SNPstream® Genotyping System:
- Download the self-extracting archive containing the methods and import files.
- Double-click the file to extract.
- Choose a directory to copy the files.
- Open the Biomek Software program.
- To import the method, go to the File menu and choose Import.
- Navigate to the directory where the extract files are located.
- Select the appropriate file that appears in the Import Method Window and click Open.
If labware and deck definition are needed follow the additional steps below:
- To import labware:
- To import the labware associated with that method go to the Project menu and select Import Project.
- Navigate to the directory where the extracted files are located.
- Select the appropriate file that appears in the Import Project Window and click Open.
- To import the deck associated with that method, go to the Instrument menu and select Import/Export Utility.
- Click Open.
- Navigate to the directory where the extracted files are located.
- Select the appropriate file that appears in the Open Import
File Window and click Open.
Note: This will be the same file that was used to import the labware into the project folder. - In the Import File Window select the deck to be imported.
- Click Import to import the deck.
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.
