Beckman Coulter Application (system and method support)
Automation of 1-3 Plates of a Beckman Coulter-Immunotech Cytokine ELISA for IL-8 on the BiomekŪ 2000 Workstation
V. Rocchi, Ph. Denizot and J. Fieschi
Immunoanalysis Department, Research & Development
Immunotech, a Beckman Coulter Company
Beckman-Coulter is the only company to provide the cytokine research community with a "Plug and Play" solution for the automation of ELISA kits for cytokines.
This solution is based on the synergy between the Immunotech ELISA portfolio and the Biomek 2000 Workstation.
All the steps of the ELISA procedure have been extensively optimized to guarantee the best throughput, reliability and sensitivity with minimal hands-on time.
The present method, suitable to run 1 to 3 plates simultaneously, was set up for a minimal robotic configuration. A fully integrated procedure is available to increase both the throughput and the walk-away time: Full automation of 1 to 10 plates of Beckman-Coulter-Immunotech cytokine ELISA on the Biomek 2000 Workstation.
Features
- Fully validated method for reliability and linearity with the existing manual procedure.
- Accurate volumes are guaranteed by the creation of "Virtual Tools" specifically calibrated to pipette Immunotech reagents.
- Processes 3 plates in less than 4 hours.
- All pipetting steps are automated, included the preparation of standards.
- Automated washing on the Biomek 2000 with Wash 8 Tool.
- Technician time limited to OD measurement on a stand-alone absorbance microplate reader.
- Minimal robotic configuration needed.
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.
