Customer Application (system support)
Automating High Content Screening Assays
Joseph Zock, Megan Weiss, Alan Stoumen, and Amy Drzal
Cellomics*, Inc. –
www.cellomics.com
Poster Abstract
The development of the High Content Screening (HCS) platform using the ArrayScan HCS System allows a new generation of multi-parameter, cell based assays to be utilized in the early drug discovery process. It is important for HCS reagents and protocols to be compatible with liquid handling and microplate preparation automation typically found in the pharmaceutical lab environment. In conjunction with our corporate partner, Beckman Coulter, Inc., we have adapted our HitKit* HCS Reagent Kits to several automation platforms including the Biomek® 2000. Additionally we have evaluated and currently use several cell washers including the MAP-C (Titertek; Huntsville, AL) and the EL405 (Bio-Tek; Winooski, VT). Examples of automated HCS will be presented along with performance and validation data.
Features
- Using standard "off the shelf" liquid handlers, one can easily automate the HitKit assays.
- Automated HCS assays reduce the variability inherent with manual processing.
- Automated HCS assays produce valid, consistent data.
- HCS can be adapted to various platforms including a stand alone Biomek 2000.
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.
