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Score the presence or absence of AFLP-generated fingerprints
The CEQ™ 8800 automatically scores the presence or absence of
amplified fragment-length polymorphism-generated fragments (dominant
scoring), making it an accurate, fast and easy-to-use tool for researches performing
AFLP fingerprinting techniques. In fact, the analysis time for 100 samples
is less than 2 minutes, versus the 40 hours often required for manual
processing.
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In this example, genomic DNA is digested with restriction enzyme(s)
such as EcoR I and Mse I, then specific adaptors are ligated to the
restriction ends of the gDNA fragments. The ligated fragments are
selectively amplified using WellRED dye labeled primers specific to the
adaptors, then separated and analyzed on a dual-plated CEQ 8800 Genetic
Analysis System.
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Quantitative analysis is possible by using an option to export the peak
heights.
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The CEQ 8000 dominant scoring algorithm automatically scores the
presence or absence of AFLP-generated fragments in binary mode (I/O)
through an integrate binning process. The dominant scoring results are
easily used for phylogenetic analysis.
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AFLP® is a registered trademark of
Keygene Corporation.
Additional Information:
Additional GenomeLab™ Information:
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