|
The ProteomeLab DU 800 is a UV/Vis spectrophotometer that has been optimized and configured for protein research. It determines protein concentrations using commercial assays and is best suited for comprehensive enzyme kinetics/mechanism studies. Other common spectrophotometric applications are also included with the system.
The total amount of protein is determined with the Protein Assay Analysis application using commercial assays such as Lowry, Bradford, Biuret, etc. that allow the quick calculation of concentrations with a standard curve.
Knowledge about the total amount of enzyme activity is often crucial in protein characterization. With the Enzyme Kinetics/Mechanism application, the speed of a reaction (rate) and the effects of a catalyst (enzyme) on the production of a new product or the disappearance of the reactant is important information. In addition, inhibitors may play a key role and can be studied with this application.
The ProteomeLab DU 800 comes with a High Performance Transport, a Peltier Temperature Controller and a Peltier temperature-controlled Auto 6-Cell Holder to give you the greatest flexibility and accurate results under highly controlled temperature conditions. No water connection is required to control the temperature of your samples or to remove the excess heat.
The hardware and software configuration of the ProteomeLab DU 800 represent a "top-of-the-line" system for protein research.
Get your results quick and easy with a fully integrated system.
|
|
Product Features
|
|
Protein Assay Analysis - Calculates protein concentrations using the Bradford, Lowry, Biuret, Direct UV, Colloidal Gold, or Bicinchoninate (BCA) methods. The user may choose to add, delete, or rerun individual standards based upon the statistical analysis of the standard curve.
Click to enlarge
Key Features
- Linear regression fit with zero or non-zero intercept.
- Non-linear (quadratic) regression.
- Use of up to 30 standards (in duplicate or triplicate, if desired).
- Optimal wavelength for assay is pre-selected.
- Individual standard selection with ability to omit or rerun standards that are out-of-range.
- Review and/or edit standard curves prior to storage and/or processing of samples.
- Zoom, trace, curve offset, autoscaling and annotation.
- Statistical analysis of sample data; variance and r2.
- Use of up to three replicates for individual samples.
- Dilution correction for each sample.
Enzyme Mechanism - Provides for rapid and easy characterization of a wide variety of enzyme reactions. The software calculates and reports Km , Vmax , kcat , and Ki as well as the Hill constant. The following functions are available: Michaelis-Menten, Lineweaver-Burk, Eadie-Hofstee, Hanes-Woolf, Hill, and Inhibitor plots.
Click to enlarge
Key Features
- Automatic blank subtraction and background correction for up to 24 blanks and samples.
- Abs/min for each sample and time point.
- Reaction rates can be determined automatically by the instrument or from user selected time points.
- Choice of rate calculation methods: linear, auto-linear, first order, and auto-first order.
- Km , Vmax , kcat , and Ki determinations by Marquardt nonlinear regression analysis.
- Plots generated include:
- Michaelis-Menten
- Lineweaver-Burk
- Eadie-Hofstee
- Hanes-Woolf
- Inhibition
- Hill
- Hill constant calculation.
- Calculation of Ki for competitive, uncompetitive, and noncompetitive mechanisms.
- Zoom, trace, curve offset, autoscaling and annotation.
A full line of modular accessories is available: ambient and temperature-controlled single and multi-position cell holders, sipper accessory, and batch sampler. Accessories to support micro-volume sampling include microcell holders for up to 12 samples, the 50 µL Microcell, the 100 µL Multi-Microcell, and the 5 µL Ultra-Microcell.
|
