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FlowCytomix kits can be run on flow cytometers with an excitation capability of 488 nm.
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Multiple Analyte Detection on Today's Flow Cytometers
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FlowCytomix kits can be run on flow cytometers with an excitation capability of 488 nm, including the automated FC 500 and XL™ Series systems, as well as the Quanta™ Family of flow cytometers. These kits use the ability of a flow cytometer to distinguish not only fluorescent intensities of microspheres, but also small size variations. Therefore, these solutions can offer 10 different bead sets, allowing the simultaneous detection of 10 different analytes.
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A New Gold Standard
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FlowCytomix kits have shown excellent correlation with ELISAs, which have long been the Gold Standard of cytokine analysis. Thus, multiplexing is quickly becoming the new
standard method for simultaneous detection of multiple cytokines and other analytes.
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This methodology provides an efficient tool, which can be used in conjunction with high volume ELISA testing to streamline assay development through standardized
testing processes.
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Sandwich assay binds specific antibody-bound beads to specific antibody-bound Biotin for a highly specific assay.
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Microsphere Technology
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FlowCytomix technology uses a sandwich immunoassay that couples the capture antibody with two different sized microspheres (4.4 and 5.5µ). Individual microsphere sets are coded using different internal fluorescent intensities. The
assay uses the same principles as an ELISA, but assesses multiple cytokines or analytes per sample.
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Complete Software Solution
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The FlowCytomix Pro software offers rapid, easy to use, analysis and reporting tools. The software creates standard curves, calculates concentrations and provides user
configurable reports and output selections in PDF and CSV formats.
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Multiplex Kits
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The FlowCytomix Multiplex kits are pre-packaged panels of analytes that address specific areas of research—ranging from immune response to cardiovascular assessment. Multiplex kits contain all the necessary reagents and analysis
software you need to perform multianalyte detection by flow cytometry.
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Simplex Kits
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Simplex kits are used for the detection of one specific analyte, and can be combined with other Simplex kits to provide a high degree of testing flexibility. These kits must be combined with the Basic Kit for complete analysis and contain the following:
- Fluorescent beads coated with corresponding antibodies
- Reference standard protein
- Biotinylated detection antibody
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Delivers Flexibility, Automation and Value
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Simultaneously quantifies 10 analytes in one small sample (25ml)
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Suitable for many automated flow cytometers, increasing speed and precision
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Flexible sample preparation in a 96-well filter plate or in tubes
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Provides ability to mix and match individual analytes for a custom multiplex assay
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Eliminates the need to maintain a separate system for bead-based analysis
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High sensitivity and broad standard ranges produce impressive results
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