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To accommodate numerous configurations, the FC 500's user changeable filters provide fluorochrome selection flexibility. Customized Photomultiplier Tubes (PMTs) detect a broad range of wavelength emissions, which can be used for virtually any dye excited by 488 or 635 nm lasers.
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Simplified Multicolor Analysis with Advanced Digital Compensation (ADC)
Beckman Coulter's ADC technology provides superior correction of spectral overlap and more accurate results. With ADC technology, biological samples can be used for automated color compensation, without the limitations of latex beads. Only Beckman Coulter flow cytometers offer patented Digital Signal Processing (DSP) for excellent linearity, drift-free amplification and color compensation.
The FC 500 with ADC also enables investigators to:
- Perform digital signal processing with 20-bit data in real-time
- Obtain fast, intuitive compensation feedback to set or fine-tune compensation manually with the QuickCOMP feature
- Increase the visualization of low intensity fluorescence populations
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Five-Color Analysis From A Single Laser Has Never Been Easier
Only the FC 500 can be used with a variety of fluorochromes to analyze five colors (FITC, PE, ECD, PC5 or APC and PC7) using either one or two lasers. Investigators could also use Beckman Coulter's IOTest multicolor reagents and drop in their choice of additional conjugated antibodies to make their own combinations.
- Performs five-dimensional automated phenotyping analysis with Prism, a unique data reduction display allowing analysis of up to 32 individual phenotypes in a single plot.
- Extends multicolor analysis to utilize the full visible emission spectra, which further boosts the level of flexibility that can be achieved
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