Microbore HPLC

Lower operational costs. More efficient interfacing with mass spectrometry. Improved sensitivity from limited sample volumes. Industry demands for benefits like these have led to using microbore chromatography for routine applications. Standard HPLC systems, however, do not adequately satisfy the need to operate with smaller bore columns at lower flow rates.

Product Features

That's why we've optimized our HPLC technology to create System Gold® Microbore HPLC with 32 Karat™ Software. There are three key steps in performing accurate and reproducible microbore chromatography. These include:

• Reproducible delivery of solvent at microbore flow rates
• Efficient mixing of solvents with minimal delay volumes
• Optimization of detector flow cell to minimize peak spreading

Beckman Coulter has taken all of these important steps into consideration in the design of this system.

	Security GLP/GMP features such as multiple levels of password protection, protected data files and audit trails ensure the integrity of your data.
	Flexibility The system is modular, combining the pump, detector and injector for reliable microbore chromatography, yet retains the ability to be used at analytical flow rates.
	Reproducibility The advanced, patented solvent delivery modules (using pressure feedback for automatic compressibility compensation) provide a constant linear velocity at microbore flow rates.
	Sensitivity The high-sensitivity diode array and UV detectors use a specially-designed low-volume flow cell to minimize peak spreading and ensure optimum sensitivity at microbore flow rates.
Click to enlarge image	The Beckman Coulter HPLC pump design allows flows to be delivered from 1 µl/min to analytical flow rates, producing consistent and accurate linear velocity to the column regardless of the solvent compressibility.
Click to enlarge image	Overlays of consecutive analyses of the peptide digests show the reproducibility of the microbore system at 50 µl/min with a 1-mm ID column. Average RSD is 0.6%.
Click to enlarge image	Shown is the comparison between the programmed gradient and the actual gradient, at 50 µl/min, from 0 to 100% B on a 1-mm ID column. The Beckman Coulter high-pressure mixing design allows for fast gradient response, yet produces superior mixing with a dynamic stir bar.