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CE-based system for the separation and characterization of proteins and
associated carbohydrates. The system is ideal for peptide mapping,
separation of proteins by molecular weight, accurate isoelectric point
(pI) determination, oligosaccharide profiling and monosaccharide
analysis. System includes a P/ACE MDQ configured with a selectable
wavelength UV/Vis (200, 214, 254 and 280 nm included) detector, UV
source optics, laser-induced fluorescence detector, 488 nm argon ion
laser module, a temperature-controlled sample storage module, and 32
Karat™ Software configured on an IBM personal computer. Installation
Qualification, Operation Qualification 1 (OQ1) and documentation to aid
in software validation is also included.
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Product Features
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Oligosaccharide Profiling
The distribution of glycans released from a glycoprotein yields a
fingerprint used in product identification and in monitoring production
processes. CE-LIF provides rapid analysis of released glycans, with
separation times typically less than 15 minutes. Extraordinary
resolution is achieved, including some positional isomers. Laser-induced
fluorescence provides high sensitivity, enabling you to simply "dilute
and shoot" samples with complex matrices
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Identity by Immunoassay
Immunoassays are commonly used in biotechnology for the detection and
quantification of host cell contaminants. The CE-LIF format eliminates
antigen immobilization and avoids many solid phase-associated problems.
Since laser-induced fluorescence detection is used, nonlabeled proteins
and peptides that do not interact with the complex are not detected,
giving clean baselines and easily interpreted electropherograms
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Glycoform Analysis
The solution-based approach of CE allows one to easily create an
environment to assess differences in a protein's glycosylation
state--directly from the protein--through the addition of salts like
borate, one can form complexes which amplify the charge state of the
glycovariants. This is the simplest approach to assessing the
heterogeneity of a glycoprotein
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Isoelectric Focusing
Identity can be partially established with the determination of a
protein's isoelectric point, while heterogeneity can be assessed with
quantitation of the different variants separated. Capillary isoelectric
focusing (CIEF) offers the advantage of analysis speed, automation, and
most important, direct quantification with on-line UV detection
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Monosaccharide Analysis
The analysis of monosaccharide composition can provide valuable
information during the initial characterization of a glycoprotein. A
clear advantage of our CE-based approach is in the specificity of the
assay. The reductive amination and open-tube format allow this analysis
to be performed directly from the glycoprotein hydrolysate--no need for
posthydrolysis cleanup. With LIF and APTS derivatization, each sugar
yields the same detector response, such that their relative quantities
can be directly compared
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Solutions for the Analysis of Macromolecules
This CD-ROM tutorial provides seminars on the development of
solution-based assays for nucleic acid, protein and carbohydrate
analyses. Animation describing these analytical processes is shown,
along with practical demonstrations on the implementation of these
assays. Our focus is to provide you with tools that improve productivity
by cutting through the complexity with simple, bold analytical solutions
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