The CEQ™ 8000 is a completely automated genetic analysis system that delivers the highest analytical performance of all commercially available sequencing platforms. The system typically sequences 200 nucleotides in less than 30 minutes, 600 nucleotides in less than 50 minutes and reads out beyond 800 quality bases with greater than 98% accuracy.
The CEQ 8000 utilizes dye terminator cycle sequencing methodology in this process. Cycle sequencing utilizes successive rounds of denaturation, annealing and extension in a thermocycler to create a linear amplification of extension products. These sequencing protocols are robust, requiring much less template than single-temperature methods. With dye terminator labeling, each of the four dideoxy terminators is tagged with a different fluorescent dye (D1 through D4). The growing chain is simultaneously terminated and labeled with the dye that corresponds to that base. With this methodology you can use unlabeled primers, all reactions are carried out in the same tube, all four colors are assessed within the same capillary and false stops go undetected because no dye is attached.
The CEQ 8000 sequence analysis software automatically calls each base and assigns a confidence value to the call to allow you to immediately assess the quality of the sequence data.
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