Agarose gel electrophoresis (AGE) has been the primary method used to assess the
homogeneity of plasmid DNA, however, this approach has some major disadvantages. The
AGE method is manual, only semi-quantitative and the assignment of bands to plasmid
structures is difficult, as the electrophoretic mobility of plasmids of different
shapes changes with the electrophoresis operating conditions A more powerful routine
technology for the quantification of plasmid forms is capillary gel electrophoresis
(CGE). This automated approach offers high resolution, high sensitivity and high
reproducibility to this analysis.
Separation of pUC19 (2.7 kbp) plasmid structures, using the P/ACE™ capillary
electrophoresis system (Schmidt et al, P/ACE Setter Newsletter, Vol. 4 Issue 2, October 2000).
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