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 Capillary electrophoresis has proven to be
the most ideal analytical tool to assess the purity of enantiomers. With this technology
it is simple to construct and modify a chiral environment, which is the key to resolving
these complex isomers.
The use of cyclodextrins for differential host-guest complexation of
enantiomers is by far the most common chiral selector and is the basis of the chiral
separation strategy that we propose.
Beckman Coulter's primary strategy focuses on the use highly sulfated
cyclodextrins (HSCDs), which are a family of three chiral reagents. This strategy first
involves screening the compound for separation using all three (a
b and g) HSCDs and then optimizing
on the reagent, which yields the best resolution. This initial screen involves the use of
a single method and has been successful in greater than 97% of the compounds that Beckman
Coulter has analyzed.
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