ProteomeLab™ IgY Single-Component Proteome Partitioning Kits

HSA BSA RSA Fibrinogen HDL IgG-Fc Transferrin The ProteomeLab IgY-R7 Single- Component Proteome Partitioning kits specifically remove any of these highly abundant proteins.

The ProteomeLab IgY Proteome Partitioning Spin Column kits are based on affinity columns using avian antibody (IgY)-antigen interactions and optimized buffers for sample loading, washing, eluting and regenerating. They are designed to work with the ProteomeLab SP sample preparation system and specifically remove a targeted protein from biological fluids such as serum, plasma, and cerebral spinal fluid (CSF) so as to enhance discovery and validation of biomarkers, drug targets, and therapeutic proteins and antibodies.

The targeted abundant protein is removed in a highly selective fashion by the immobilized specific IgY capture reagent when complex biological samples are passed through the column. Selective immunoaffinity partitioning provides an enriched pool of lower abundant proteins for downstream proteome analysis, as well as enabling the investigator to analyze isoforms of the captured protein and trace materials that are physiologically associated with the target protein. Both the unbound material in the flow-through fraction and the highly abundant protein in the bound/eluted fraction can be collected and further fractionated with the ProteomeLab PF 2D System. Our technology uses physiological buffers for binding and washing, and avoids urea and other chaotropic agents for elution that can precipitate at low temperature.

Each kit provides enough reagents for 200 cycles, with a plasma or serum capacity per cycle of 20 µL to 125 µL depending on the species and kit selected.

Cleaner Capture

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Since the polyclonal IgYs are avian, the Fc region of the antibody does not bind mammalian complement factors, Rheumatoid Factor, IgM, Fc receptor and Protein A or G, a known issue related to capture with mammalian antibodies. Coupled with high specificity across multiple epitopes, you get very specific binding across species while achieving very clean capture. Partitioning efficiency is typically greater then 99.5%. The SDS-PAGE on the left highlights whole serum in lane #1, serum with just albumin partitioned out by Proteome-Lab IgY-HSA SC chemistry in lane #2, and the isolated/partitioned albumin captured cleanly in lane #3.

ProteomeLab IgY Single-Component Spin Kit Contents & Ordering Information