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High Performance Gel-Forming Cocktail
Sample Holding Diagram
Extreme Counting Conditions
Ready Gel is well suited to experiments that generate samples with:
- pH values less than 3 or up to 10
- Salt concentrations up to 2M
- Sample volume equal to cocktail volume
- Organic solvents
The cocktail can eliminate the uncertainty associated with cloudy counting conditions. Samples are solubilized into a clear solution or, in the case of large volumes, into a stable gel.
Consistent Sample Capacity
Stability of clear liquid and gel phases for a variety of common sample types have been confirmed by a less than 3% change in counting efficiencies over a minimum 72-hour period at the temperature range of 10°C to 30°C. In practice, phase stability for both regions may last for months. Holding capacities for these samples are shown in the above phase diagram. Many other cocktails have unstable gel regions above 25°C. Ready Gel maintains gel stability to 30°C.
Counting Efficiency
Counting efficiency for Tritium is virtually unsurpassed by any other high sample content cocktail. See the following table for counting efficiencies with common samples.
Typical Counting Efficiencies for Tritium
Using Ready Gel
Standard vials with 1 mL Sample in 10 mL cocktail
Mini-vials with 1 mL sample in 5 mL cocktail
|
| Sample |
Standard Vials
%Efficiency Appearance |
Mini Vials
%Efficiency Appearance |
| Water |
40.8 |
Clear |
32.2 |
Gel |
| PBS Buffer |
40.7 |
Clear |
32.8 |
Gel |
| 1.0M NaCl |
39.8 |
Clear |
N/A |
N/A |
| 0.1M HCl |
39.3 |
Clear |
30.0 |
Gel |
| 0.1M NaOH |
40.2 |
Clear |
33.0 |
Gel |
| 8.0M Urea |
39.8 |
Clear |
30.8 |
Gel |
| 20% Sucrose |
41.1 |
Clear |
31.7 |
Clear |
Low Activity and Low Level Samples
With low activity or low level samples, efficiency and throughput can be increased by taking advantage of the high sample holding capacity of Ready Gel. For example, if you count to a 2% 2-sigma error, you must accumulate a total of 10,000 counts. To do this faster, simply add more sample. Although the counting efficiency may decline as more sample is added, you will actually obtain more counts and so reach the preset error more quickly. When you count to a preset time, greater sample volume will mean that you will accumulate more counts and therefore have better statistical counting accuracy.
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