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BTS-450 is a quaternary ammonium hydroxide (0.5N) in toluene. It rapidly and economically digests tissues and tissue homogenates. Up to 0.45 mL of water can be dissolved in 1 mL BTS-450. Just follow a simple series of steps to solubilize and count:
- Weigh sample in a glass vial (see table below)
- Add 1 mL BTS-450 and digest sample at 40°C - 50°C until tissue dissolves
- Decolorize with 30% H2O2
- Add 70 µL glacial acetic to eliminate chemiluminescence
- Add 10 mL Ready Organic™ and count
Sample Table
Typical Samples Solubilized by 1 mL BTS-450
| Sample |
Amount |
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Sample |
Amount |
| Arteries |
20 - 100 mg |
|
Liver |
80 - 100 mg |
| Brain |
80 - 130 mg |
|
Muscle |
100 - 220 mg |
| Cartilage |
20 - 55 mg |
|
Nerve Cells |
50 - 100 mg |
| Cornea |
40 - 160 mg |
|
Pancreas |
50 - 140 mg |
| Heart |
100 - 120 mg |
|
Spleen |
50 - 140 mg |
| Intestine |
80-100 mg |
|
Stomach |
80 - 100 mg |
| Kidney |
80 - 100 mg |
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Other uses of BTS-450 include solubilization of blood, serum and feces.
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