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  System Gold® HPLC --
  Systems, Pumps and Modules

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System Gold® HPLC systems incorporate the latest technological advances in hardware design. The solvent delivery modules are built with the digitally controlled rapid refill pump design with automatic compressibility compensation that has received multiple patents. These features produce smooth flow from microbore to semi-preparative flow rates, and the high-pressure mixing design eliminates the need for constant helium sparging or degassing. The pumps are combined with high-sensitivity UV/VIS and diode array detectors and autosamplers with automation capability to build HPLC systems that have a reputation for the best performance, ease of use and troubleshooting, and longevity due to a solid and reliable design.

The HPLC systems are capable of standalone control via an easy-to-program front panel on the pump module or they can be controlled with 32 Karat™ chromatography software which offers data collection and analysis as well.

Gold HPLC systems are available with a variety of options, including stainless steel or non-metallic systems, built-in programmable 4-way solvent selector valves, built-in programmable column switching valves, and microbore to semi-preparative flow rates. This makes it possible to select a system that will best fit the needs of any laboratory.

All HPLC systems which include a minimum of a pump and detector include installation and basic training by qualified Field Service Engineers.

All HPLC systems and modules include a full year in-lab warranty. Details are on quotations and the warranty statement.


 

System Gold BioEssential HPLC Package 

These systems include everything needed to start performing HPLC except solvents, column, and a printer. These systems include the pump, detector, injector, and data system as indicated. Also included are all cables, drip tray, column mounting bracket, solvent reservoir kit, and test kit, as well as installation and training. Due to the advanced pump design, an on-line helium sparging or degassing apparatus is not required for optimum operation. A one-time vacuum degassing prior to loading solvents can be done if desired. Piston wash is a standard feature on all System Gold pumps.

BioEssential HPLC systems do not come standard with Validation (IQ/OQ) or multi-use features. These features can be purchased as additions to the BioEssential Package (see below).

Each BioEssential HPLC Package is delivered with the following parts:
Part No. Description
728786 Bottle Rack
726555 Bottle with Cap
238447 Test Mix
713060 32 Karat™ Workstation
713064 32 Karat™ software package
718149 System Gold HPLC manual
977493 CRT flat panel color 15" (monitor)
718133 Basic training insturment workbook
243533 Ultrasphere® ODS Pre-Column - 4.6 mm ID, 45 mm length.
538747 Column Mounting Assembly HPLC
239310 Remote Interface Module - Provides interface to input/output connector on System Gold® modules. For fraction collectors, integrators, and other relay-controlled hardware.
718120 User's Guide
728125 Injector Organizer Tray - With mounting plate for manual injector.
142485 508 Remote Start Cable - Included with all systems that include a pump and detector.

System Gold BioEssential HPLC Packages 

Part No. Description
285540 System Gold HPLC BioEssential with Binary Gradient 125 Pump and UV/VIS 166 Detector - Includes workstation and manual injector kit.
285541 System Gold HPLC BioEssential with Binary Gradient 125 Pump and UV/VIS 166 Detector - Includes workstation and 508 Autosampler.
285542 System Gold HPLC BioEssential with Binary Gradient 125 Pump and UV/VIS 166 Detector - Includes workstation and 508 Autosampler with cooling.
285525 System Gold HPLC BioEssential with Binary Gradient 125 Pump and Diode Array 168 Detector - Includes workstation and manual injector kit.
285526 System Gold HPLC BioEssential with Binary Gradient 125 Pump and Diode Array 168 Detector - Includes workstation and 508 Autosampler.
285527 System Gold HPLC BioEssential with Binary Gradient 125 Pump and Diode Array 168 Detector - Includes workstation and 508 Autosampler with cooling.
285543 System Gold HPLC BioEssential with Binary Gradient 126/8 Solvent Selection Pump and UV/VIS 166 Detector - Includes workstation and manual injector kit.
285544 System Gold HPLC BioEssential with Binary Gradient 126/8 Solvent Selection Pump and UV/VIS 166 Detector - Includes workstation and 508 Autosampler.
285545 System Gold HPLC BioEssential with Binary Gradient 126/8 Solvent Selection Pump and UV/VIS 166 Detector - Includes workstation and 508 Autosampler with cooling.
285528 System Gold HPLC BioEssential with Binary Gradient 126/8 Solvent Selection Pump and Diode Array 168 Detector - Includes workstation and manual injector kit.
285529 System Gold HPLC BioEssential with Binary Gradient 126/8 Solvent Selection Pump and Diode Array 168 Detector - Includes workstation and 508 Autosampler.
285530 System Gold HPLC BioEssential with Binary Gradient 126/8 Solvent Selection Pump and Diode Array 168 Detector - Includes workstation and 508 Autosampler with cooling.
285557 System Gold HPLC BioEssential Nonmetallic with Binary Gradient 125 Pump and UV/VIS 166 Detector - Includes workstation and manual injector kit.
285551 System Gold HPLC BioEssential Nonmetallic with Binary Gradient 126/8 Solvent Selection Pump and UV/VIS 166 Detector - Includes workstation and manual injector kit.
285556 System Gold HPLC BioEssential Nonmetallic with Binary Gradient 125 Pump and Diode Array 168 Detector - Includes workstation and manual injector kit.

Computer-Controlled “Add-On” Systems 

These systems require connection to a computer that is running Beckman Coulter chromatography software. To build a complete HPLC system, combine one of these systems with the desired injector and computer system from the following pages. These are called “add-on” because they are ready to add on to existing Beckman Coulter chromatography workstations such as System Gold, Gold Nouveau, and 32 Karat. If there is an open system slot on the workstation, then simply selecting one of these systems along with an injector will provide an additional HPLC system.
Part No. Description
267867 Microbore Binary Gradient with UV/VIS 125S/166
285404 Microbore Binary Gradient with Diode Array 125S/168
267852 Binary Gradient with UV/VIS 125/166
267856 Binary Gradient with Diode Array 125/168
267853 Binary Gradient/8 Solvent Selection with UV/VIS 126/166
267857 Binary Gradient/8 Solvent Selection with Diode Array 126/168
267868 Semi-Prep Binary Gradient with UV/VIS 125P/166P
239879 Semi-Prep Binary Gradient/8 Solvent Selection with UV/VIS 126P/166P
149808 Semi-Prep Binary Gradient/8 Solvent Selection with Diode Array 126P/168
267858 Binary Gradient with UV/VIS 125NM/166NM
267866 Binary Gradient with Diode Array 125NM/168NM
267869 Semi-Prep Binary Gradient with UV/VIS 125NMP/166NMP
267859 Binary Gradient/8 Solvent Selection with UV/VIS 126NM/166NM
267863 Binary Gradient/8 Solvent Selection with Diode Array 126NM/168NM
267835 Semi-Prep Binary Gradient/8 Solvent Selection with UV/VIS 126NMP/166NMP

Front Panel Control "Add-On" Systems 

These systems include pumps with System Control Centers which allow true single-point programming of the pumps, UV/VIS detectors, and autosamplers. A computer is not required to control the HPLC system, making these systems ideal for connection to centralized chromatography data systems, integrators, or chart recorders. To build a complete HPLC system, combine one of these systems with the desired injector from the following pages. These systems can also be “added-on” to existing Beckman Coulter chromatography workstations, such as System Gold, Gold Nouveau, and 32 Karat, and controlled by the software.
Part No. Description
267883 Microbore Binary Gradient with UV/VIS 127S/166S
267854 Binary Gradient with UV/VIS 127/166
267855 Binary Gradient/8 Solvent Selection with UV/VIS 128/166
267880 Semi-Prep Binary Gradient with UV/VIS 127P/166P
267885 Semi-Prep Binary Gradient/8 Solvent Selection with UV/VIS 128P/166P
267864 Binary Gradient with UV/VIS 127NM/166NM
267882 Semi-Prep Binary Gradient with UV/VIS 127NMP/166NMP
267865 Binary Gradient/8 Solvent Selection with UV/VIS 128NM/166NM
267887 Semi-Prep Binary Gradient/8 Solvent Selection with UV/VIS 128NMP/166NMP
267851 Isocratic with UV/VIS 118/166

Injectors and Autosamplers 

A manual injector or autosampler is added to Build-to-Order HPLC Systems to complete the hardware configuration.

Manual Injectors

The manual injector kits are complete for connection to a System Gold HPLC. Included are the Rheodyne injector, mounting bracket, 50 µL syringe, and integrated remote start switch with 2 cables to initiate system start when injector is actuated.
Part No. Description
239890 Rheodyne 7725 Manual Injector Kit (Stainless Steel)
267816 Rheodyne 9725 Manual Injector Kit (Non-Metallic)

Autosamplers 

The 508 autosamplers can be controlled by either a front panel control pump or a computer workstation with 32 Karat™ software. All feature 96 sample capacity with full, partial, or no sample loss injection modes. Options are Peltier sample cooling and integrated programmable column switching valves. Each version is available in stainless steel or non-metallic (PEEK).

When used with Nouveau software Version 1.7 or 32 Karat™ software and the proper tray configurations, the 508 autosamplers can be used for sample dilution, standard addition, reagent addition, with mixing and delay programming.
Part No. Description
Stainless Steel
142490 508 Autosampler, Basic
142492 508 Autosampler with Sample Cooling
142492 508 Autosampler with Column Switching
142493 508 Autosampler with Both Sample Cooling and Column Switching
Non-Metallic
142495 508 NM Autosampler, Basic
142496 508 NM Autosampler with Sample Cooling
142497 508 NM Autosampler with Column Switching
142498 508 NM Autosampler with Both Sample Cooling and Column Switching
Accessories
143243 10 mL Vial Tray Segment - REQUIRED FOR REAGENT ADDITION AND DILUTION.
143223 Microvial Tray Set - Provides 160 positions for microvials.
143224 4 mL Vial Tray Set - Provides 72 positions for 4 mL vials.
143225 10 mL Vial Tray Set - Provides 32 positions for 10 mL vials.
143217 Syringe, 1000 µL
143218 Syringe, 100 µL
143219 Syringe, 500 µL
729821 Syringe, 250 µL (Supplied Standard on 508)
729861 Buffer Tubing, 500 µL - For 250 and 100 µL syringes (supplied standard on 508).
729862 Buffer Tubing, 1000 µL - For 500 µL and 1000 µL syringes.
149865 Upgrade Kit, 507e to 508 - Includes installation.

Data Chromatography Workstation and Software 

32 Karat™ Chromatography Software is a newly developed package for instrument control, data collection and analysis that runs under the Windows 2000 operating system. This full 32-bit software incorporates modern ease-of-use functionality combined with all of the features needed for research, biotechnology, or pharmaceutical laboratories. Each 32 Karat™ workstation is capable of running up to four Gold HPLC systems with multiple channels. 32 Karat™ software has been developed using documented processes and is fully validated at Beckman Coulter.

Each 32 Karat™ package is shipped with one IEEE board and one Gold board, ready for two HPLC system controls (one if diode array system), and two P/ACE MDQ System controls. One additional Gold Board can be installed to allow two additional HPLC system controls. In addition, Analog-to-Digital boards are available for data collection from non-Beckman Coulter detectors.

The 32 Karat™ Workstation includes a computer platform that has been validated by Beckman Coulter for the operation of 32 Karat™ software. Due to the rapid technological changes in computer hardware, please contact your sales representative for details on the validated computer platforms for software-only purchases.
Part No. Description
285418 32 Karat™ Workstation
Includes 32 Karat™ software installed on an IBM Pentium computer with Windows 2000 software. Six hours of user training are included. Contact Beckman Coulter sales for current computer configuration.
Includes 32 Karat™ Controller, software, flatpanel CRT and 32 Karat™ Valdiation Pack.
285417 32 Karat™ Software Only
Includes 32 Karat™ software only. Six hours of user training is included. Requires a compatible computer platform.
Includes 32 Karat™ software, HPLC PCI-Com Board, and Validation Pack.

32 Karat™ Workstation Options 

Part No. Description
713116 32 Karat™ Reprocessing Software (For 32 Karat™ Software version 3.0 or 3.1)
Allows reprocessing of data collected on 32 Karat™ at a remote or network computer.
(Does not allow instrument control).
715063 32 Karat™ Reprocessing Software (For 32 Karat™ Software version 5.0 or greater)
713118 32 Karat™ Uninstall Disks (quantity 3)
713020 Analog to Digital Data Collection Board
Allows data collection from any non-Beckman Coulter detector with an analog output.
For HPLC systems with software versions of 3.1 or greater.
142485 508 Remote Start Cable - Included with all systems that include a pump and detector.
Required for automated start of A/D Board (713020) and 508 autosampler.
721290 PCI Gold Board
721272 PCI Gold Board Cable

32 Karat™ Software Migration Packages 

32 Karat™ Software is a newly designed software package optimized for controlling Gold HPLC systems and running on the Windows 2000 operating system. Users of System Gold Chromatography software (which runs on the DOS operating system) and Gold Nouveau Software (which runs on the DOS and Windows 3.1 operating systems) can easily migrate their existing Gold HPLC hardware to 32 Karat. However, migrating to 32 Karat™ software requires moving to a new operating system (Windows 2000) which in turn will require moving to a new computer in the vast majority of cases. The Migration Packages offer favorable pricing for 32 Karat™ software for users of previous Beckman Coulter software.

The 32 Karat™ Workstation includes a computer platform that has been validated by Beckman Coulter for the operation of 32 Karat™ software. Due to the rapid technological changes in computer hardware, please contact your sales representative for details on the validated computer platforms for software-only purchases.

Migration Packages include six hours of training on the new 32 Karat™ software.
Part No. Description
285419 32 Karat™ Migration Workstation
Includes 32 Karat™ software installed on an IBM Pentium computer with Windows 2000 software. Contact Beckman Coulter sales for current computer configuration. Includes Program CD, E-manuals CD, 32 Karat™ Tutorial, Basic Training Workbook, IEEE board, Gold communication board, 32 Karat™ migration guide, Key return, firmware.
285420 32 Karat™ Migration Software
Includes 32 Karat™ software only. Six hours of user training is included. Requires a compatible computer platform as described above. The 32 Karat™ software can be user installed easily onto a computer that meets the requirements as outlined above. However, it is possible that hardware or memory conflicts could occur which could require additional troubleshooting. Installation help is available from Beckman Coulter Field Service at the normal hourly rate.
715068 32 Karat™ Software Upgrade
For users with 32 Karat™ only.

Compliance Services 

Beckman Coulter offers a variety of products and services to help users comply with regulations such as GLP and GMP. Training by qualified Field Service Engineers assists in compliance with training regulations. All HPLC systems are designed, manufactured, and delivered under a documented quality system that assures quality products. 32 Karat™ Software is designed and validated using accepted processes to support compliance of computer systems, including new FDA Part 11. Instrument and software features are designed to help in developing validated methods and supporting the validity of data through system suitability or standard checks.

Many labs also follow various forms of Equipment Qualification (EQ). Various levels of Installation Qualification (IQ) and Operational Qualification (OQ) are offered to assist with these processes.

Contact Beckman Coulter sales (see last page) for more information concerning compliance with FDA and other regulations.

OQ services do not include non-Beckman Coulter HPLC modules.
Part No. Description
144867 Installation Qualification (IQ)

For original start-up of new system. Correct shipment, installation, documentation and safety review are documented on an IQ Data Sheet.
721112 HPLC OQ 1 (no charge with purchase of instrument system)
Basic performance verification in the installed environment and OQ1 Data Sheet.
721113 HPLC OQ 2, No Support Agreement
Basic performance verification, preventive maintenance, and software verification.
Documented on OQ2 Data Sheet.
726139 HPLC Certification (OQ 3)
Complete modular HPLC system qualification using test instruments and standards with NIST traceability, software verification, and full documentation.
149919 32 Karat™ Validation Pack
285531 Validation Software IQ, OQ, Security
This includes all IQ/OQ materials and the following:
726139 HPLC Certification (OQ 3)
718157 32 Karat™ Software
285532 Workstation Multi-User / CE Control
This option enables customers to use the 32 Karat™ workstation with multiple HPLC/CE systems.

System Gold® Accessories 

Part No. Description
149859 Alltech 631 Column Heater
142489 Model 234 Programmable Gradient Temperature Column Heater
142482 Remote Start Communications Cable
For use with Model 234 column heater (for remote method start).
267850 System Gold® HPLC Starter Kit
Includes 4 reservoirs with caps, solvent rack, drip tray with valve mounting plate, and system test kit.
239286 Injector Interface Cable
For System Gold® modules; can be used with 502/507/507e autosamplers and manual injector (one required for each System Gold® module; not required for software-operated systems with 502/507/507e).
239018 Interface Cable
1 meter (links system component to system component).
239019 Interface Cable
3 meters (links system component to system component).
239020 Interface Cable
20 meters (links system component to system component).
Injector Organizer Tray
With mounting plate for manual injector.
726555 Bottle with Cap
726546 Bottle Cap
238447 Test Mix
239257 System Test Kit
Includes 4.6 x 45 mm C18 column, test sample and instructions.
239915 Tubing Kit
Detector to fraction collector.
142128 Low Dead Volume Back Pressure Regulator, 60 psi
285546 SC-100 Fraction Collector Kit
Includes cable and tubing kit. Remote Interface is included in each pump+detector package.
239310 Remote Interface Module
Provides interface to input/output connector on System Gold® modules. For fraction collectors, integrators, and other relay-controlled hardware.
142190 SC100 Fraction Collector
Collects fractions by time, drop, pumped volume, or choose from three different peak collect routines. Comes standard with 13 mm tube rack.
142182 Cable to Connect SC100 to Gold® HPLC System
142177 Rack for 18 mm Test Tubes (Capacity 72)
142178 Rack for 1.5-mL Microfuge Tubes (Capacity 60)
142179 Rack for 2 Conventional or Deep-Well 96-Well Plates
235268 Column Adapter
FPLC column-to-System Gold® (requires 2 per column).

System Gold® HPLC Pumps 

System Gold HPLC modules are available individually. In most cases, however, it is recommended that a pump/detector combination is ordered from page 1 or 2 of this price list. This assures that a properly matched system with the appropriate accessories will be delivered.

SS = Stainless Steel, PEEK = Non-Metallic

125 Pump Family: Dual gradient high pressure mixing (requires PC control) 

Part No. Description
728394 125 Analytical Pump, SS
728745 125P Preparative Pump, SS
728395 125NM Analytical Pump, PEEK
728746 125NMP Preparative Pump, PEEK
728399 125S Analytical Pump, Microbore, SS

126 Pump Family: Dual gradient high pressure mixing with dual 4-way solvent selection (requires PC control) 

Part No. Description
728396 126 Analytical Pump, SS
728747 126P Preparative Pump, SS
728397 126NM Analytical Pump, PEEK
728748 126NMP Preparative Pump, PEEK

127 Pump Family: Dual gradient high pressure mixing (stand alone with front panel control) 

Part No. Description
728127 127 Analytical Pump, SS
728135 127P Preparative Pump, SS
728630 127NM Analytical Pump, PEEK
728627 127NMP Preparative Pump, PEEK
728137 127S Analytical Pump, Microbore, SS

128 Pump Family: Dual gradient high pressure mixing with dual 4-way solvent selection 

Part No. Description
728128 128 Analytical Pump, SS
728129 128P Preparative Pump, SS
728629 128NM Analytical Pump, PEEK
728628 128NMP Preparative Pump, PEEK

118 Pump: Isocratic with 4-way solvent selection (stand alone with front panel control) 

Part No. Description
728118 118 Analytical Pump, SS

Parts for 116/118/125/126/127/128 Pumps

Microbore HPLC Pump Accessories
 

Part No. Description
240090 Micro Mixer Kit - For 126 (bolt-on).
147880 Static Mixer, 150 µL volume
147885 Micro Tubing Set - Converts non-micro pump tubing to micro.
149855 Micro Conversion Kit (for new-style, bolt-on mixer pumps)
Includes bolt-on micro mixer, static mixer, tubing kit, and instructions.

Other Pump Accessories and Parts 

Part No. Description
239400 Customer Service Kit
240714 Piston, Analytical
241037 Piston Seal, Analytical
241017 Piston Seal, Analytical PEEK
241018 Piston Seal, Preparative PEEK
237163 Liquid Head, Analytical
238610 Solvent Inlet Filter, Tube End
240790 Solvent Inlet Filter, In-Line
237115 Priming Valve
239491 Mixer Disc
239323 Stir Bar, Upper
239324 Stir Bar, Lower
236107 Pulse Damper
239968 Filter Assembly, In-Line, High-Pressure
239970 Replacement Filter, 2 µm
240101 Preparative Liquid Head Kit
For 125 and 116/126 with S/N >369; two required for each 125 and 126 pump.
238446 Preparative Liquid Head Kit
For 116/126 SDS with S/N <369 (requires two for 126).
240715 Piston, Preparative
728773 Piston Wash Seal, Low Pressure, Preparative
240720v Inlet Check Valve, Complete Assembly
240620 Inlet Check Valve, Cartridge
239423 Inlet Check Valve, Body
240721 Outlet Check Valve, Complete Assembly
240621 Outlet Check Valve, Cartridge
240061 Outlet Check Valve, Body
240619 Check Valve Filter Replacement Kit
240624 Check Valve Cartridge Removal Tool
241578 Inlet Check Valve, Cartridge, PEEK
241422 Inlet Check Valve, Body, PEEK
241579 Outlet Check Valve, Cartridge, PEEK

 

Part No. Description
241417 Outlet Check Valve, Body, PEEK
 



 

 
 
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