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Nucleic Acid

eCAP™ dsDNA 20,000 Kit
 
Part No. 477486

The eCAP™ dsDNA 20,000 Kit provides coated capillarires with all the necessary reagents, standards, and instructions to perform large fragment (up to 20,000 bp) nucleic acid separations. For analysis of double-stranded DNA with linearity from 1,000 to 20,000 base pairs. Includes application disk, coated capillaries (2), replaceable gel, and standards to perform at least 100 runs.
Part No. Description
477477 eCAP™ DNA Capillary - 100 µm ID, 65 cm total length (quantity 1).
477485 eCAP™ dsDNA 20,000 Gel Buffer (pack of 3)
477483 eCAP™ Orange G Reference Marker
241524 eCAP™ Orange G Reference Marker

eCAP™ dsDNA 1000 Kit 
Part No. 477410

For DNA fragment pattern recognition, quantitation of PCR* fragments, and molecular sizing determinations. Typical resolution for fragments less than 400 base pairs is 5-15. Molecular size linearity from 100 to 1000 base pairs. The kit includes a coated capillary and replaceable gel, test mix and an internal standard to perform at least 100 runs. For analysis of double-stranded DNA with linearity from 100 to 1,000 base pairs. Includes application disk, coated capillaries (2), replaceable gel, and standards to perform at least 100 runs.
Part No. Description
477477 eCAP™ DNA Capillary • 100 µm ID, 65 cm total length (quantity 1).
477628 eCAP™ ssDNA 1000 Gel Kit (pack of 3)
477414 eCAP™ dsDNA 1000 Test Mix

Flourescene Intercalator 

Part No. Description
477409 eCAP™ EnhanCE Dye 0.5 mL

eCAP™ ssDNA 100-R Kit 
Part No. 477480

For analysis of single-stranded DNA with linearity from 10 to 100 bases. Includes application disk, coated capillaries (2), replaceable gel, and standards to perform at least 100 runs. UV detection is required to analyze unlabeled and standard oligonucleotides. Urea denaturant is included separately, enabling a naturing replaceable gel, if desired. For analysis of single-stranded DNA from 10 to 100 bases.
Part No. Description
477477 eCAP™ DNA Capillary - 100 µm ID, 65 cm total length (quantity 1).
477621 ssDNA 100-R Gel Kit
338481 eCAP™ ssDNA 100 Buffer Kit - Includes Tris borate buffer (2), Urea, 7 M (2).

Small Molecules

P/ACE™ System MDQ Highly Sulfated Cyclodextrins
 

Recommended for use only with P/ACE System MDQ because of the high current running conditions.
Part No. Description
149869 Highly Sulfated Cyclodextrin Trial Kit - Consists of 5 mL each of the three HS Cyclodextrins; Test Mix; Marker; Conditioning Solution; and Phosphate Buffer.
713348 HS Alpha Cyclodextrin (5 mL)
713349 HS Alpha Cyclodextrin (100 mL)
713331 HS Beta Cyclodextrin (5 mL)
713347 HS Beta Cyclodextrin (100 mL)
713350 HS Gamma Cyclodextrin (5 mL)
713351 HS Gamma Cyclodextrin (100 mL)
713330 HS Cyclodextrin Test Mix
713328 PTS Marker (1 mL @ 10 mMolar)
713333 Capillary Conditioning Solution (10 mL)
477422 Phosphate Buffer, pH 2.5 (qty. 50 mL)

P/ACE™ System MDQ Chiral Methods Development 
Part No. 501300

To be used for rapid development in the analysis/quantitation of chiral compounds. Repackaged for System MDQ. Consists of 2 neutral capillaries, high pH buffer, low pH buffer, adjustable pH buffer, beta-cyclodextrin, gamma-cyclodextrin, HP-beta-cyclodextrin, and DM-beta-cyclodextrin, Test Mix, Methods Development Guide, Chiral Methods Development Flow Chart, and Buffers Dilution Guide.
Part No. Description
477441 ProteomeLab™ Neutral Coated Capillary - 50 µm I.D.x 45 cm total length.
477449 Chiral Kit High pH Buffer (500 mL)
477448 Chiral Kit Low pH Buffer (500 mL)
477447 Chiral Kit Adjustable pH Buffer (500 mL)
477469 Chiral Kit Test Mix (1 mL)
477437 Beta-cyclodextrin (25 g)
477438 Gamma-cyclodextrin (25 g)
477467 Dimethyl-beta-cyclodextrin (25 g)
477470 Hydroxypropyl-beta-cyclodextrin (25 g)

eCAP™ Amine Capillary Methods Development Kit/Small Molecules 
Part No. 477430

When basic compounds “stick” to the slightly anionic surface of a fused silica capillary, the amine capillary may be the solution. The surface of the amine capillary has been modified with a polyamine to producea strong cationic charge which reverses the direction of electroosmotic flow and minimizes the potential for basic analyte interactions with the capillary wall. Expect reduced separation times, improved resolution and efficiency and simplified methods development. The kit contains two amine capillaries and regenerator solution, buffers, micellar agents, test mix and a marker to perform at least 200 runs. For basic compounds that require a fast EOF and a cationic capillary surface.

Kit includes:
Part No. Description
477431 eCAP™ Amine Capillary - 50 µm ID, 65 cm total length (quantity 1).
477432 eCAP™ Amine Capillary - 75 µm ID, 65 cm total length (quantity 1).
477425 Acetate Buffer, pH 4.5
477426 MES Buffer, pH 6.0
477427 Tris Buffer, pH 8.0
477422 Phosphate Buffer, pH 2.5 (qty. 50 mL)
477423 Phosphate Buffer, pH 7.0
477428 TTAB (Micellar Agent)
477429 Triton X-100, Micellar Agent
477433 Amine Regenerator Solution
477421 Small Molecule Test Mix
477434 Neutral Marker

eCap™ Carbohydrate Labeling Kit

Carbohydrate Labeling and Analysis Chemistries
 
Part No. 477600

Requires 488 nm Argon LIF Dectector on P/ACE 2000-5000 or P/ACE MDQ

The eCAP™ Carbohydrate Labeling Kit provides a highly automated separation and detection of N-linked oligosacchrides. Kit includes the necessary labeling reagent (APTS), eCAP™ N-CHO capillary (2), buffers, standards, and test mix.

Kit includes:
Part No. Description
477601 ProteomeLab™ N-CHO Capillary 50 µm I.D. x 65 cm total length
477623 N-Linked Gel Buffer
501309 APTS Labeling Dye, 2 x 5 mg
725898 APTS-M Grade, 21 mg - For monosaccharide analysis
 

* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffmann-LaRoche, Ltd.

For Research Use Only; not for use in diagnostic procedures.



 

 
 
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