|
|
| |
|
DNA Synthesis Supplies: Quality and Convenience You Expect
Beckman Coulter's proven CED-phosphoramidite synthesis chemistry and packaging conveniences improve efficiencies and deliver higher yields. UltraFAST DNA Synthesis Technology reduces cleavage and deprotection time to just ten minutes and improves step-wise coupling efficiency. Phosphoramidite packaging includes activated molecular sieves to assure dryness and coupling efficiency. The cleavage and deprotection chemistry is provided in kits with syringes and vials that are compatible with Beckman Coulter reactor columns.
Quality and Convenience
Each reagent and phosphoramidite bottle is color-coded and clearly labeled to correspond directly with its position on the instrument. Augmented by dC-acetyl columns and amidites, Beckman Coulter's traditional CED-phosphoramidite chemistry significantly reduces the time it takes to acquire high-quality oligonucleotides.
- Economical Mono-Pak and Binary-Pak™ phosphoramidites include molecular sieves to assure dryness, and a minimum of four weeks life on the instrument.
- Single pore-size reactor columns used for both short and long oligonucleotides feature a zero dead volume design that minimizes reagent consumption.
- Convenient cleavage and deprotection kits include UltraFAST chemistry that cleaves and deprotects in just ten minutes.
The nomenclature for the phosphoramidites and their protecting groups is as follows: Bz - benzoyl, Ac - acetyl, iBu - isobutyrl. Bz protects adenosine and standard cytidine. Ac protects UltraFAST cytidine. iBu protects guanosine. Thymidine and Inosine do not have protecting groups.
|
|
Reactor Columns, 1/Pack
|
|
Single pore-size glass column supports with the first nucleotide covalently attached. Column threads
directly onto the Oligo Series Synthesizers and are guaranteed to be suitable for the synthesis of
both short and long oligonucleotides.
|
|
|
|
|
|
Reactor Columns, 30 nmole, 1/Pack
|
|
|
| Part No. |
Description |
| 337719
|
Reactor Columns, dA(Bz), 30 nmole
|
| 337793
|
Reactor Columns, dC(Ac), 30 nmole
|
| 337721
|
Reactor Columns, dG(iBu), 30 nmole
|
| 337722
|
Reactor Columns, T, 30 nmole
|
|
|
|
|
|
Reactor Columns, 200 nmole, 1/Pack
|
|
|
| Part No. |
Description |
| 337723
|
Reactor Columns, dA(Bz), 200 nmole
|
| 337795
|
Reactor Columns, dC(Ac), 200 nmole
|
| 337725
|
Reactor Columns, dG(iBu), 200 nmole
|
| 337726
|
Reactor Columns, T, 200 nmole
|
|
|
|
|
|
Binary-Pak™ CED-Phosphoramidites
|
|
The phosphoramidite and acetonitrile are premeasured and packaged in a ready-to-mix bottle
configuration containing activated molecular sieves, to provide extended life of at least four weeks
on the instrument.
|
|
|
|
|
|
|
0.25 gram Binary-Paks
|
|
|
| Part No. |
Description |
| 337737
|
Binary-Pak™ CED-Phosphoramidites, dA(Bz), 0.25 gram/5 mL Acetonitrile
|
| 337790
|
Binary-Pak™ CED-Phosphoramidites, dC(Ac), 0.25 gram/5 mL Acetonitrile
|
| 337739
|
Binary-Pak™ CED-Phosphoramidites, dG(iBu), 0.25 gram/5 mL Acetonitrile
|
| 337740
|
Binary-Pak™ CED-Phosphoramidites, T, 0.25 gram/5 mL Acetonitrile
|
|
|
|
|
|
0.5 gram Binary-Paks
|
|
|
| Part No. |
Description |
| 337743
|
Binary-Pak™ CED-Phosphoramidites, dA(Bz), 0.5 gram/10 mL Acetonitrile
|
| 337791
|
Binary-Pak™ CED-Phosphoramidites, dC(Ac), 0.5 gram/10 mL Acetonitrile
|
| 337745
|
Binary-Pak™ CED-Phosphoramidites, dG(iBu), 0.5 gram/10 mL Acetonitrile
|
| 337746
|
Binary-Pak™ CED-Phosphoramidites, T, 0.5 gram/10 mL Acetonitrile
|
|
|
|
|
|
Mono-Pak™ CED-Phosphoramidites
|
|
The phosphoramidite is packaged in a bottle containing activated molecular sieves for extended life
of at least four weeks on the instrument. Requires acetonitrile (P/N 338161) for 0.5 g and 1.0 g sizes,
respectively.
|
|
|
|
|
|
|
0.5 gram Mono-Paks
|
|
|
| Part No. |
Description |
| 337704
|
Mono-Pak CED-Phosphoramidites, dA(Bz), 0.5 gram.
|
| 337705
|
Mono-Pak CED-Phosphoramidites, dC(Ac), 0.5 gram.
|
| 337706
|
Mono-Pak CED-Phosphoramidites, dG(iBu), 0.5 gram.
|
| 337707
|
Mono-Pak CED-Phosphoramidites, T, 0.5 gram.
|
|
|
|
|
|
1.0 gram Mono-Paks
|
|
|
| Part No. |
Description |
| 337708
|
Mono-Pak CED-Phosphoramidites, dA(Bz), 1.0 gram.
|
| 337709
|
Mono-Pak CED-Phosphoramidites, dC(Ac), 1.0 gram.
|
| 337710
|
Mono-Pak CED-Phosphoramidites, dG(iBu), 1.0 gram.
|
| 337711
|
Mono-Pak CED-Phosphoramidites, T, 1.0 gram.
|
|
|
|
|
|
Other Synthesis Reagents
|
|
|
| Part No. |
Description |
| 338161
|
Base Diluent, Acetonitrile, 45 mL in 100-mL Bottle
Used with Mono-Pak phosphoramidites at 50 mg/mL concentration. Includes activated
molecular sieves to ensure dryness. <10 ppm water.
|
| 337731
|
DNA Synthesis Reagent Kit
Recommended for Oligo 1000 only. Includes: P/N 337732 Oxidize, 337733 DEblock, 337734
CAP 1, 337735 CAP 2, premeasured for simultaneous depletion and replenishment when
used with the Oligo 1000.
|
| 338284
|
Activate
Used to convert the stable phosphoramidite into reactive species (0.5 M Tetrazole/
Acentonitrile).
|
| 338293
|
Oxidizer
Used to oxidize the phosphorous on each cycle (3% I2 in THF, pyridine, water).
|
| 338248
|
Deblock
Used to remove the trityl protecting group on the phosphoramidite (3% Trichloroacetic
Acid in Dichloromethane).
|
| 338282
|
Cap 1 (Cap A Replacement)
Automatically combined with Cap 2 reagent during synthesis to terminate incomplete
reaction sequences (10% Acetic Anhydride in Tetrahydrofuran, lutidine).
|
| 338295
|
Cap 2 (Cap B Replacement)
Automatically combined with Cap 1 reagent during synthesis to terminate incomplete
reaction sequences (17% N-methyl-Imidizole/Tetrahydrofuran).
|
|
|
|
|
|
Post-Synthesis Reagents and Supplies
|
|
|
| Part No. |
Description |
| 607819
|
UltraFAST Cleavage and Deprotection Kit (set of 80)
Provides cleavage within 5 minutes at room temperature and deprotection within 5 minutes at
65°C. Includes 80 disposable syringes, 80 vials specially adapted with O-rings compatible with
ammonia methylamine (AMA), and sufficient AMA to process 80 oligonucleotides synthesized
at either 30 or 200 nmole scale or 40 oligonucleotides synthesized at 1,000 nmole scale.
|
|
|
|
|
|
Replacement Parts and Supplies
|
|
|
| Part No. |
Description |
| 964338
|
10 Liter Waste Bottle
Polyethylene, fluorinated, split- and puncture-resistant bottle to use with Oligo 1000M
or with the Oligo 1000 in place of the 4 liter bottle. Meets DOT-2T (Department of Trans-
portation) specificationsto transport most hazardous reagents.
|
| 607909
|
Shield, Acetonitrile - Plastic acetonitrile safety container. Requires P/N 607838 Cap.
|
|
|
|
|
|
Replacement Parts
|
|
|
| Part No. |
Description |
| 523198
|
RS-232 Cable - Straight-through, 25-pin serial cable (M-F) for X-Y plotter; 9 feet long.
To link instrument to a personal computer for downloading sequences and for
developing user-defined methods.
|
|
|
|
|
|
