P/ACE™ MDQ Nucleic Acid Analysis Kits - Consumables

Related Product Information

The eCAP ssDNA Kit is designed for the analysis of oligonucleotides, providing a direct quantitative tool for quality control. This chemistry utilizes linear polyacrylamide in solution to form a sieving gel, which provides single base resolution of oligonucleotides between 10 and 100 bases. The neutral capillary provided with the kit has a stable, physically bonded polyacrylamide coating, which eliminates electroosmotic flow (EOF) and suppresses interactions of the sample with the capillary wall. This chemistry utilizes a standard Tris-Borate EDTA buffer system including high concentrations of urea to prevent secondary structure formation. Applications of this kit have included DNA and RNA probes, ribozymes and anti-sense phosphorothioate oligonucleotides. The system is compatible with both UV and LIF detection systems to include the assessment of fluorescent labels.

Kit includes coated capillaries (2), replaceable gel, and standards to perform at least 70 runs. Urea denaturant is included separately, enabling a naturing replaceable gel, if so desired.

Analysis of 22-mer oligonucleotide; 300 V/cm and 30 C.

Separation of synthetic 17-mer oligonucleotide with the trityl group attached to the 5' end. Insert shows high-speed separation of the same oligonucleotide accomplished using reverse injection from the short side of the capillary.

The eCAP dsDNA 1000 Kit is designed for the analysis of double-stranded DNA fragments from 72 to 1000 base-pairs. This chemistry utilizes linear polyacrylamide in solution to form a sieving gel. A neutral capillary provided with this kit has a stable, physically bonded polyacrylamide coating which eliminates electroosmotic flow (EOF) and suppresses interactions of the sample with the capillary wall. This chemistry utilizes a standard Tris-Borate EDTA buffer system and allows the addition of intercalating dyes like ethidium bromide or our proprietary EnhanCE™ to improve resolution and increase sensitivity. This kit is ideal for the analysis of PCR* products which can be analyzed directly with no sample clean-up. Applications of this kit have included gene expression analysis (competitive PCR), direct hybridization viral load determination (no amplification) and genotyping experiments with RFLPs, STRs and AmFLPs. The system is compatible with both UV and LIF detection, but LIF is recommended for most applications.

Kit includes: (2) 100 µm I.D. neutral capillaries (DNA), (3) vials of replaceable polyacrylamide gel, (1) test mix consisting of Hae III restriction digest f174 DNA containing 11 fragments from 72 bp to 1,353 bp, and instructions and methods for system set-up and analysis. For LIF applications, we recommend the use of the intercalating dye EnhanCE.

Separation of an Hae III restriction digest of fX174 DNA using LIF detection. Pressure injection for 10 seconds (10 µg/mL sample).

CE-LIF analysis of DNA products generated by competitive RNA-PCR* of cytochrome P450-1A1 mRNA in dioxin-treated HepG2 cells. Target sequence is 367 bp; competitor is 228 bp; CF=carboxyfluorescein marker; IS=328 bp internal standard.

The eCAP dsDNA 20,000 Kit is designed for the analysis of double-stranded DNA fragments from 1,000 to 20,000 base-pairs. This chemistry utilizes linear polyacrylamide in solution to form a sieving gel. A neutral capillary provided with this kit has a stable, physically bonded polyacrylamide coating which eliminates electroosmotic flow (EOF) and suppresses interactions of the sample with the capillary wall. This chemistry utilizes a standard Tris-Borate EDTA buffer system and allows the addition of intercalating dyes like ethidium bromide or our proprietary EnhanCE, to improve resolution and increase sensitivity. This kit is ideal for the analysis of large PCR* products or plasmids and plasmid digests which can be analyzed directly with no sample clean-up. The system is compatible with both UV and LIF detection.

Kit includes: (2) 100 µm I.D. neutral capillaries (DNA), (3) vials of replaceable polyacrylamide gel, (1) test mix consisting of Hind III restriction digest of l DNA. Contains 8 fragments from 125 bp to 23,130 bp, and instructions and methods for system set-up and analysis. For LIF applications, we recommend the use of the intercalating dye EnhanCE.

Before and after separations of DNA fragments derived from the complete EcoR1 digestion of a 12 Kbp plasmid vs. the undigested plasma itself.