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Purification & Clean-Up

PCR Purification

Agencourt AMPure XP

The Agencourt AMPure XP system is a highly efficient, easily automated PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration, Agencourt AMPure XP can be easily used in manual and automated 96- or 384-well formats.

 

Overview

Agencourt AMPure XP Features
 
  • High recovery of amplicons, greater than 100 bp
  • Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants
  • Stable PCR products post-cleanup: No PCR degradation after storage at 4° C for seven days
  • Efficient recovery of double stranded and single stranded DNA templates
  • Consistent recovery throughout the kit’s 12 month shelf life
  • Faster manual and automated processing as compared to traditional post-PCR cleanup methods

Downstream Application Techniques

PCR, genotyping (SNP detection) fragment analysis, sequencing (Sanger & Next Generation), cloning, primer walking


Process Overview


1. PCR reaction 2. Binding of PCR amplicons to magnetic beads 3. Separation of PCR amplicons bound to magnetic beads from contaminants 4. Washing of PCR amplicons with Ethanol 5. Elution of PCR amplicons from the magnetic particles 6. Transfer away from the beads into a new plate


High Recovery

The Agencourt AMPure XP purification system recovers amplicons greater than 100 bp with consistency and high reproducibility. The recovery efficiency of the Agencourt AMPure XP kit for small and large amplicon sizes is superior to the traditional filtration PCR cleanup methods (Figure 1).


Walk-away Automation

Agencourt AMPure XP post-PCR cleanup has been automated for process scalability on three Beckman Coulter liquid handlers, the Biomek 4000, NXP and FXP. LIMS compatible automation functionality is aided by a user-friendly interface equipped with a quick startup menu, deck setup walk-through menu, a method recovery menu and a procedure menu.
 

Recommended Accessories 
 
Part No. Description
 A32782  Agencourt SPRIPlate Super Magnet Plate
 A29165  Agencourt SPRIPlate 384 Magnet Plate
 

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