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Software

Simply, efficiently and quickly analyze multicolor, multiparametric data from flow cytometers of today and tomorrow.

Kaluza for Gallios Acquisition Software

Analytical muscle for your collected data

With four possible laser configurations scalable to 10 colors, sophisticated forward and side scatter detectors, state-of-the-art optics, and other innovative features, Gallios delivers powerful, versatile performance. And Kaluza provides the analytical muscle to make the most of the data generated.

Download our free offline software which allows users to author Kaluza protocols and worklists for the Gallios flow cytometer on their own computers.

Features

More experimenting, less frustration


Features like these make Kaluza easy to use.

  • No database to impact performance—save your files to where you specify
  • Auto-layout feature for easy plot creation and sizing, zoom in/out, and guided drag and drop
  • Visual management tools to help you understand what is happening to your experiment and cytometer
  • No user profiles or login required to run acquisition
  • Set up a 10 color experiment in seconds
  •  


See data with fresh eyes through innovative new plot types
Visualize and manipulate your data with multiple plot types in addition to traditional scatter, histogram and contour plots.

  • Quickly and easily classify cells with the tree plot
  • Radar plot with gates–
    • Why see 2D when you can see 3D?
  • Overlay histograms to compare multiple plots in one view
  • Overlay density and contour plots

Analyze MILLIONS of cells, not thousands
Manipulate as many as 10,000,000 events in real time! Interact with your data in new ways. Watch plots and statistics change as you modify gates with Kaluza patent-pending technology.

  • Work with large data sets in real time
  • Perform complex analysis with ease
  • Maximize your investment in computing processors

Experience the quality difference
Uncompromising quality and reliability for multi-file, high-content data analysis–

  • Worry-free analysis
  • If you crash it, we want to know!

Learn more

Intelligent design for data exploration
Experience the performance difference of Kaluza

User Interface

Easy to learn and easy to use at all experience levels

Kaluza for Gallios is built on the strong, innovative foundation of Kaluza Analysis Software. With Kaluza you can power through data sets containing multiple colors, plots and events with speed and precision. The result: your flow cytometry research can find that extra gear to get you to your objective sooner.

 

 

Acquisition controls are right where you need them. Adjust flow rate, compensation and voltage with ease.

 

 

Visual management allows you to see what is in the carousel and watch data come in real time. Use the guided compensation wizard to help you create a compensation experiment.

Computer Specs

Kaluza for Gallios ships with a Windows 7 PC with the following specifications:

Specification Value
Operating System Win7Pro64ME8.0-English
1st Graphic Card QuadroK600 1GBDLDVI-I+DP W7ME8
Processor IntelXeon E5-2620v2 2.1GHz 6c
Hard Drive Controller Adapter Intel SATA HDD support1-3 HDDs
RAID MI SATA RAID 1-2 HDDs req
1st HDD 3.5" 500GB SATA HDD | 7200 rpm
2nd HDD 3.5" 500GB SATA HDD | 7200 rpm
Keyboard USB Full Kybd Win8 English103P
Mouse Lenovo Opt Wheel Mouse USB
Optical Drive 16x DVD ROM W7 ME8.0
1st Memory 4GB ECC PC3 1333MHz UDIMM
2nd Memory 4GB ECC PC3 1333MHz UDIMM
3rd Memory 4GB ECC PC3 1333MHz UDIMM
4th Memory 4GB ECC PC3 1333MHz UDIMM
Audio Card Integrated Audio
Ethernet Integrated Ethernet + Dual NIC
Service 3_3 ONSITE_WW
Pub PUB_English
Line Cord LineCord_US

Kaluza FAQ

What is Kaluza?

Kaluza Software is a revolutionary flow cytometry analysis software solution designed for high content data. Kaluza provides visualization tools, speed and innovative simplicity to the flow community. With features designed to make your life in the lab easier, Kaluza aims to provide a greater understanding of the data generated by modern cytometers. Kaluza employs patent pending technology that allows for real time analysis of high content files, allowing you to spend your valuable time on discovery.

 

Is Kaluza compatible with the FCS files from my system?

Kaluza is compatible with files that comply with the FCS standard through the most recently released standard. The software has been tested against a large repository of these files, but there are also tools available within Kaluza to ensure that parameters are loaded properly (i.e. linear vs. log).

 

Where can I purchase the Kaluza software?

You can purchase Kaluza by selecting the product(s) from the Products tab or speaking with your Beckman Coulter representative.    

 

Where did Kaluza get its name?

Theodor Franz Eduard Kaluza (November 9, 1885 - January 19, 1954) was a German mathematician and physicist known for the Kaluza-Klein theory involving field equations in five-dimensional space. His idea that fundamental forces can be unified by introducing additional dimensions re-emerged much later in string theory. His theories attempted to rationalize complex multidimensional space which is analogous to the challenges faced with when analyzing complex flow cytometry data.

 

How is Kaluza licensed?

Kaluza is licensed through the use of a hardware key, commonly known as a romlock or dongle. These keys provide easy portability of software from one computer to another and for analysis on your laptop when you’re on the move.

 

How does the network license work?

The network license provided with Kaluza is based on the number of concurrent sessions running Kaluza. An unlimited number of computers can have the software installed. The network license will control the maximum number of users actively logged into the network key. The key management software can be easily accessed through a web-based program.

 

What are the recommended computer specifications?

Kaluza employs patented technology that revolutionizes the speed at which data is analyzed. As with all software, the speed at which it operates is dependent on the specification of your hardware as well as the size and complexity of the data being handled. Please see more detailed information in the Computer Specifications section.

 

How do I get more performance from my current system?

Kaluza provides an Event Sampling Mode that allows you to optimize performance with large event files on sub-optimal computer workstations. It can be accessed from the Kaluza Options dialog via the main launch icon. This feature optimizes performance when moving gates or updating compensation by setting the application to temporarily decrease the number of events that appear on the plot.

 

What operating systems are compatible with Kaluza?

Kaluza v1.5 was validated on Windows 7 SP1, Windows 8.1 and Window 10 operating systems.  Ad hoc testing was performed on Windows XP 32-bit SP3, Windows Vista 32-bit SP2 operating systems .Kaluza is also compatible with MAC hardware using those operating systems under dual-boot programs such as "Bootcamp". 

OS X systems running in a virtual machine environment with Windows such as "Parallels" and "VirtualBox" has not been verified and has been known to show visual glitches. Also, the demo mode in virtual machines has been disabled.

 

What new features have been added to Kaluza 1.5? 

Many new features were added to version 1.5 of Kaluza. Please view those features on the NEW with Kaluza 1.5 tab.

 

What language packages are available for Kaluza 1.5?

Currently Kaluza is available also in Simplified Chinese and Japanese. Other language packages will be posted here as they become available.

 

How do I upgrade to 1.5?

A free of charge download is available here.  Your existing dongle will work with this build of Kaluza.

 

Will Kaluza v1.5 work with my Kaluza for Gallios Acquisition software?

Yes, Kaluza v1.5 will work with Kaluza Acquisition software however some functionality in Kaluza v1.5 was not updated in Kaluza for Gallios. This may cause some cases on incompatibility.

In a single display, the tree plot easily renders what would otherwise require dozens of plots, gates and regions. Each “tree branch” represents a unique phenotype that can be used as a gate for further exploration.

Training Courses

File/Info Title Ref. Number
Kaluza Flow Cytometer Training Modules

Videos

  • Kaluza for Gallios - Using the Simulator with Modern Browser

    Click to play  

  • Kaluza for Gallios Compensation Tutorial - Creating a Compensation Matrix

    Click to play  

  • Kaluza for Gallios Compensation Tutorial - Creating a Compensation Worklist

    Click to play  

  • Kaluza for Gallios Compensation Tutorial - Using the Simulator with a Legacy Browser

    Click to play  

  • Kaluza Software: Experience the performance difference of Kaluza

    Click to play  

    Kaluza´s revolutionary speed makes analysis of multiple datasets as simple as drag & drop. Patented data processing technology enables simultaneous analysis of many large files.
  • Kaluza Software: Extended Features for Kaluza Analysis

    Click to play  

  • Kaluza Software: Getting to Know Your Kaluza Acquisition Software

    Click to play  

  • Kaluza Software: Ins and Outs of Kaluza Analysis

    Click to play  

  • Kaluza Software: Intelligent design

    Click to play  

    Kaluza is designed to simply, efficiently and quickly analyze multicolor, multiparametric data from cytometers of today and tomorrow. Kaluza´s intelligent design provides an intuitive environment for data exploration.
  • Kaluza Software: Launch

    Click to play  

  • Kaluza Software: Parametric Visualization

    Click to play  

    Kaluza´s innovative multiparametric plots revolutionize the way multicolor data is analyzed. Multidimensional visualization tools can significantly reduce time required for complex data analysis.
  • Kaluza Software: Performance

    Click to play  

    Kaluza’s revolutionary speed makes analysis of multiple datasets as simple as drag & drop. Patented data processing technology enables simultaneous analysis of many large files.
  • Kaluza Software: You Have Your Data, Now What? Setting Up Statistics

    Click to play  

  • Kaluza Tutorial: Attributes Pane

    Click to play  

    The Kaluza Attributes pane displays the data file’s Parameters, Compensation, and Color Precedence details.

    Click on the Parameters tab to view the data file’s Parameter Names and Descriptions. Click on the chevron to Show...

  • Kaluza Tutorial: Creating Plots

    Click to play  

    Plots are easily added to a dataset by clicking on the desired plot type from the Plots and Tables Ribbon tab. To assign parameters to the plot, simply click on the parameter hyperlink on each axis. Any plot type may be dragged and dropped from...

  • Kaluza Tutorial: Opening data files

    Click to play  

    Data files may be opened in Kaluza by:

    Selecting the Open Files button in the center of the Kaluza workspace. Alternatively, you may open files from the Application Menu located at the upper left of the Kaluza workspace. Also from the...

  • Kaluza Tutorial: Overlay Plot

    Click to play  

    An overlay plot is a combination of multiple histogram plots displayed in a single plot. It is a highly visual data comparison tool and up to 8 histograms may be added for display. Several formatting tools are available through the Plot Radial...

  • Kaluza Tutorial: Radial Menus

    Click to play  

    Radial menus provide quick access to a variety of editing tools in Kaluza.  Simply right click on a plot, gate, Plot Sheet or Report sheet to view the Radial menus.  As you hover over any Radial menu icon, a menu appears allowing you...

  • Kaluza Tutorial: Tree Plots

    Click to play  

    When working with high color in flow cytometry, the number of data plots increases significantly with each color added to the analysis. A Tree Plot is a useful data comparison tool that can condense data from up to 28 bivariate plots. It is...

  • Webinars & Symposiums

  • Data Flow Rethinking Data Analysis for Flow Cytometry - Kaluza 1.1

    Sharlene Wright, Beckman Coulter

    Click to play  

    Designed to quickly and efficiently analyze multi-color, multi-parametric high content data generated by today's flow cytometers, the revolutionary Kaluza analysis software provides novel visualization tools, real time speed and powerful...
  • DuraClone - A New Frontier in Dry Reagents

    Aprajita Priyadarshini, Sr. Applications Scientist, Beckman Coulter

    Click to play  

    DuraClone is an innovative approach for dry reagents for flow cytometry. The proprietary process results in a product that is consistent from tube to tube and lot to lot and eliminates the need for cold chain storage. The process is distinct...

  • Microvesicle Detection and Cell Sorting

    Vasilis Toxavidis, MChS, HCPR Resource Director, Beth Israel Deaconess Medical Center Flow Cytometry Core Facility, Harvard Stem Cell Institute John Tigges, Beth Israel Deaconess Medical Center Flow

    Click to play  

    There is great interest in both medical and scientific communities in submicron cell derived particles, termed microparticles or microvesicles. A great difficulty in this field, however, has been the optimization and standardization of...

  • Stem Cells: Identification & Isolation through High-Speed Cell Sorting

    E. Michael Meyer, Research Scientist Manager, Flow Cytometry Facility University of Pittsburgh Cancer Institute

    Click to play  

    The discussion of Stem Cells can cover a range of topics and cell types; hematopoietic progenitor cells, mesenchymal, adipose, and even "cancer" stem cells are just few of the types of stem cells that we work with on a daily basis...

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