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PerFix-nc (no centrifuge assay kit)

PerFix-nc Kit (no centrifuge assay kit)

How much time do your technicians spend on intracellular staining?

Is intracellular staining disrupting your workflow with multiple centrifugations and upfront surface staining steps?

PerFix-nc from Beckman Coulter can help you streamline your procedure and gain some of that time back, with advanced, innovative intracellular staining chemistry that delivers excellent reproducibility for results you can trust. Stain in a single step. Detect surface and intracellular antigens simultaneously, with fewer or no wash steps, 45 minutes total time, and just 15 minutes of hands-on time.

Now available for in vitro diagnostic use in the United States, Canada, European Union, and other geographies. PerFix-nc carries both CE-IVD and IVD labeling, ensuring regulatory compliance.

Features

PerFix-nc kit lyses red blood cells and induces permeability in the cytoplasmic membrane of leukocytes for intra- and extracellular staining for flow cytometry analysis.

Beckman Coulter provides several hundred of IVD and CE-IVD single colors reagents, multi-color cocktails, kits, assays and software to fit the needs of clinical customers from sample preparation to data analysis.

PerFix-nc kit provides the fastest commercially available procedure for simultaneous detection of surface & intracellular antigens. Beckman Coulter’s state of the art chemistry allows removal of wash steps, thus streamlining your workflow while increasing your assay reproductibility.

PerFix-nc

Simplified workload

  • Less than 15 minutes hands-on time
  • Intracellular staining as simple as surface staining
  • No centrifuge, No wash straight procedure
  • Room temperature (no water bath or ice required)
  • Automation-ready solution
  • One kit, flexible protocols, multiple applications

Proven performance

  • No wash and less preparation steps leads to better reproducibility
  • Compatible with all Beckman Coulter dyes
  • Retains versatility, with optional wash
  • Adaptable to specific antigen detection requirements
  • Remarkable cell recovery and gate purity
  • Enhances the signal-to-noise ratio for intracellular staining

Fast • Simple • Robust • Reproducible

 

Reliable intracellular screening of Hematological Malignancies on both Whole Blood (WB) and Bone Marrow (BM) specimens

 
Whole Blood sample Bone Marrow sample
CD45-Krome Orange
CD45-Krome Orange - Whole Blood sample CD45-Krome Orange - Bone Marrow sample
MPO-FITC
MPO-FITC - Whole Blood sample MPO-FITC - Bone Marrow sample
CD3-ECD / CD79a-PE
CD3-ECD / CD79a-PE - Whole Blood sample CD3-ECD / CD79a-PE - Bone Marrow sample
 

Get the most information from inside the cells. PerFix-nc combines ease-of-use, reliability and reduced hands-on time to optimize Leukemia & Lymphoma diagnostics. Its innovative chemistry provides optimal staining for the major intracellular markers such as MPO, CD79a, TdT, Kappa & Lambda and others.



 

On both WB or BM, light scatter characteristics are well preserved with optimal staining patterns. Patented chemistry reduces background, improving sensitivity of your intracellular assays.



 

High Fix No-wash PerFix-nc procedure on whole blood and bone marrow samples. Staining with CD45-Krome Orange, MPO-FITC and CD3-ECD/CD79a-PE.

 

One kit with flexible protocols to fit the needs of your major clinical applications which require intracellular staining.

Example of Kappa/Lambda staining

Example of Kappa/Lambda staining

Low Fix Wash PerFix-nc procedure on whole blood sample. Staining with Lambda-PE and Kappa-FITC. Gating on lymphocytes (left) and on CD19 positive B cells (right). Excellent discrimination of Kappa-positive versus Lambda-positive cells.

Example of Intracellular TdT staining on normal and pathological blood samples

Normal blood sample Pathological blood sample
Example of Intracellular TdT staining

Low Fix No wash PerFix-nc procedure on normal and pathological blood samples. Staining with TdT-FITC. Gating on CD45 positive leucocytes for both normal and pathological samples. Easy detection of challenging intracellular antigens.

 
Order Information: PerFix-nc Kits

IVD & CE 75 tests Part # B31167
IVD & CE 150 tests Part # B31168
Component B31167 B31168
1PerFix-nc Buffer 2 - Fixative Reagents 1 vial 2 vials
2PerFix-nc Buffer 2 - Permeabilizing Reagents 1 vial 2 vials
3PerFix-nc Buffer 3 - Final 10X Solution 1 vial 2 vials
Storage at room temperature (18-25°C)

Videos

  • PerFix-nc - no centrifuge assay kit

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