This website would like to remind you: Your browser (IE8) is out of date.Update your browser for more security, comfort and the best experience on this site.
×
Quote Request

PerFix EXPOSE

PerFix EXPOSE (Phospho-Epitopes Exposure kit)

The PerFix EXPOSE Kit (Phospho-Epitopes Exposure kit) is a Fast & Easy Procedure for Cell Signaling. It consists of three ready-touse reagents, and one solution requiring a 20-fold dilution before use. Its purpose is to induce permeability in the cytoplasmic and nuclear membranes of leucocytes or human cell lines for the detection of phosphorylated intracellular antigenic determinants by means of dyes or fluorochrome-conjugated antibodies.

Features

PerFix EXPOSE Kit can be used to prepare biological samples for analysis by flow cytometry. It has been developed to enhance the signal-to-noise ratio of most intra-cellular phospho-epitopes stainings and to simplify the workload necessary for the sample preparation. Accurate detection of both intracellular and extracellular epitopes is obtained, while:

  • There are only two washing steps through the procedure.
  • There is no methanol treatment, and therefore no ice incubation required.
  • A unique procedure can be applied to all phospho-epitopes.
  • Several surface markers can be added together with the intracellular markers and incubated simultaneously.
  • Total duration of the procedure and total workload are similar to current procedures for surface staining (about 1 hour).
PerFix EXPOSE - 12 minutes hands-one time only!

§ 47 to 70 minutes according to the number of tubes to perform and the incubation time recommended for the antibodies used.

The PerFix EXPOSE Kit (Phospho-Epitopes Exposure kit) is a Fast & Easy Procedure for Cell Signaling. Its purpose is to induce permeability in the cytoplasmic and nuclear membranes of human leucocytes or cell lines for the detection of phosphorylated intracellular antigenic determinants by means of dyes or fluorochrome-conjugated antibodies. PerFix EXPOSE Kit can be used to prepare biological samples for analysis by flow cytometry.

Videos

  • PerFix EXPOSE - Phospho Epitopes Exposure Kit

    Click to play  

  • Products

    Instruments - Clinical Diagnostics

    Show: 25 50 100 items per page.

    Info
    Part No.
    Name
    Lasers
    Detectors
    Reg
    Price
    Buy

    Instruments - Research and Discovery

    Show: 25 50 100 items per page.

    Info
    Part No.
    Name
    Lasers
    Detectors
    Reg
    Price
    Buy

    Reagents - Clinical Diagnostics

    Show: 25 50 100 items per page.

    Info
    Part No.
    Name
    Species
    Form
    Clone
    Reg
    Price
    Buy

    Reagents - Research and Discovery

    Show: 25 50 100 items per page.

    Info
    Part No.
    Name
    Species
    Form
    Clone
    Reg
    Price
    Buy

    B26976

    PerFix EXPOSE, 75 test, RUO

    RUO

    $410.00

    For research use only. Not for use in diagnostic procedures.
    Kaluza Questions & Concerns
    RNACleanXP Certificate of Analysis
    CleanSeq Certificate of Analysis
    AmpureXP Certificate of Analysis
    Request the Quote
    Reach Out
    Reach Out Part 2
    Download Resource
    Service Return Authorization Request
    Kaluza 30-Day Trial