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Krome Orange

Krome Orange

Exclusively from Beckman Coulter, our new organic fluor, Krome Orange™ dye, maximizes the potential of your violet laser. Krome Orange dye has been optimized for superior optical performance including high signal to noise ratios and minimal intra-laser compensation requirements.

Krome Orange dye has been optimized for superior optical performance including high signal to noise ratios and minimal intra-laser compensation requirements.

  • Narrow, well separated emission profiles
  • Excited by violet laser; optimally detected using a 550/40 filter
  • Virtually no excitation detected using a 488 nm laser

Features

Fluorophore Molar Extinction Coefficient
(M-1-1CM-1)
Absorbance Maximum (nM) Emission Maximum (nM)
Krome Orange™ 17,665 398 528
Pacific Blue™ 46,000 410 455
Pacific Orange 24,500 400 551
AmCyan NA 458 489
Horizon V500 NA 415 500

Krome Orange dye is at least as bright as Horizon™ V500 dye and can provide more than twice the population separation obtained with Pacific Orange™ dye conjugates and with little compensation versus Pacific Blue™ dye.

  • Less compensation than Horizon V500 dye versus Pacific Blue dye
  • Unlike AmCyan, essentially no compensation versus 488-excited channel
  • Better population resolution than Pacific Orange dye

Krome Orange dye is optimally suited to deliver exceptional performance on instruments equipped with a violet laser.


Krome Orange - Dye Excitation

Dye Excitation

Krome Orange dye is optimally suited to deliver exceptional performance on any flow cytometer equipped with a 405nm violet laser.

Krome Orange dye excitation curve (dashed black line); emission curves for Krome Orange dye, Pacific Orange dye, AmCyan, and Horizon V500 dye.


Krome Orange - Staining Patterns

Staining Patterns

CD45 example histogram overlays of the fluorescence excitation and emission spectra showing Krome Orange staining patterns relative to other violet laser dyes.


Krome Orange - Histograms

Histograms

Example histograms showing conjugate staining patterns gated on monocytes except for CD14 overlay showing monocytes and lymphocytes. Histograms are labeled with staining index (SI) values.


Citation PubMed - U.S. National Library of Medicine NIH
J Immunol Methods. 2011 Sep 30;372(1-2):42-51. Epub 2011 Jul 14. The new violet laser dye, Krome Orange, allows an optimal polychromatic immunophenotyping based on CD45-KO gating.

Webinars & Symposiums

  • Krome Orange: A Novel Orange-Emitting Dye for Use in Multi-Color Flow Cytometry Panels Using Violet Diode Laser Excitation

    Laura Nieto Gligoroski, Departement Analyse Cellulaire, Beckman Coulter Immunotech

    Click to play  

    Violet diode lasers have become common excitation sources on flow cytometers, but the advantages of this laser are constrained by limited choices for organic, violet-excited fluorochromes. We have developed a novel violet-excited organic dye...
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