Spatially Separated Laser Options (Beam Spot Size: 5 μm x 80 μm)
Fixed integrated optics and quartz flow cell design with >1.3 numerical aperture. Flow Cell dimensions: 420 μm x 180 μm internal diameter
Forward Scatter Detection
Proprietary Axial Light Loss (ALL) sensor system using silicon photodiodes with built in 488/8 μm band pass filter.
Fluorescence and Side Scatter Detection
Fluorescence and side scatter light delivered by fiber optics to Avalanche Photo Diode detector arrays. Proprietary design ensures high performance, high efficiency, low-noise signal detection. Emission profiles are collected using reflective optics and single transmission band pass filters.
Violet Side Scatter Configuration
Option to configure Avalanche Photo Diode detector array to collect side scatter signal from Violet (405 nm) laser. The configured channel (VSSC) can be utilized to better resolve nanoparticles below 200 nm size threshold.
For detection channel off of the 405, 488, and 638 nm laser, CytExpert QC automation pass/fail criteria is rCV ≤5.0%. For detection channels off of the 808 nm laser, the criteria is ≤ 7.0%.
Blue (488 nm) Side Scatter Resolution
Violet (405 nm) Side Scatter Resolution (VSSC)
Forward and Side Scatter Resolution
Scatter performance is optimized for resolving human lymphocytes, monocytes, and granulocytes as well as nanoparticles.
Single Tube Format: < 1.0%
Plate Loader Format: < 0.5%
FITC: <30 molecules of equivalent soluble fluorochrome (MESF-FITC)
PE: <10 molecules of equivalent soluble fluorochrome (MESF-PE)
The CytoFLEX Flow Cytometer is capable of achieving 3% rCV with alignment verification particles capable of rCVs <3%.
Nominal Acquisition Rate
30,000 events per second with 15 parameters
Software capability to modify window extension parameter and to control abort rate during high event rate signal processing
Fully digital system with 7 decade data display
Pulse area, height for every channel, width for one selectable channel
Ultra-low Pressure Peristaltic Sheath and Sample Delivery System
Low maintenance system
Sheath Pump tubing and Sample Pump tubing can be replaced by the user (no service visit required)
Sample Flow Rates
Fixed Flow Rates: 10, 30 and 60 μL/min
Custom Flow Rate Control mode from 10 to 240 μL/min in 1 μL increments.
Gravimetric calibration for absolute counts within CytExpert Software.
Standard 4 L tanks
Optional 10L cubitainers
Automated Maintenance Functions
System Startup, Sample Mixing, Backflush, Prime, Shutdown, Deep Clean
Sample Input Formats
5 mL (12 x 75 mm) polystyrene and polypropylene tubes
1.5 mL and 2 mL microcentrifuge tubes
Plate Loader Formats
96-well Standard Flat, U and V bottom plates
The CytExpert software is a full-feature software package that controls the instrument's operation, collection of experiment data, and analysis of the results.
Three different installation modes are available, depending on the level of security required. The Default installation requires no user login. For multiuser instruments, the User Management installation requires user login and contains features for role management. Electronic Records Management installation provides tools that facilitate compliance with 21 CFR Part 11, Electronic Records and Electronic Signatures.
An API (Application Programming Interface) is available and allows external software to perform operations such as running methods and for basic control of the plate loader.
Daily QC beads or any other reference material that is relevant for your application may be used as the standardization sample to set target values and calibrate the gain settings automatically. The Standardization Target Library stores a variety of application target files which are linked to the reference material lot numbers.
English and Chinese
Windows® 7 Professional 64-bit
Windows® 8 Professional 64-bit
Windows® 10 Professional 64-bit
CPU: Intel® I3 @ 2.9 GHz 1 Gigabit Ethernet port
RAM: 4 GB 2 USB 3.0 ports
Storage: 256 GB 4 USB 2.0 ports
Automatic Full matrix compensation
Manual full matrix compensation
Novel Compensation Library: store fluorescent spillover values of dyes to easily determine the correct compensation matrix with new gain settings
Import/export compensation values between experiments
Absolute linear gain amplification enables the use of compensation settings between experiments and sample types
Dimensions (W x D x H)
|Cytometer (with or without Plate Loader)
||Tanks and Holder
|42.5 cm x 42.5 cm x 34 cm
||14 cm x 35.6 cm x 35.6 cm
|16.7 in x 16.7 in x 13.4 in
||5.5 in x 14.0 in x 14.0 in
Cytometer: 23.4 kg / 51.6 lbs
Cytometer with Plate Loader: 28kg / 61.7 lbs
Voltage: 100–240 V
Power: 150 -250 W
Operating Temperature Non-Condensing
15-27 °C, 59-80.6 °F