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Instrumentation

Beckman Coulter offers a wide range of innovative cell sorters and analyzers to expand your clinical and research flow cytometry capabilities.

Gallios

Analytical excellence

The GalliosTM Flow Cytometer research system delivers analytical excellence by coupling extraordinary sensitivity, resolution and dynamic range with high-speed data collection. Along with the unprecedented detection capabilities of the instrument, the Gallios includes easy-to-use software and automation to facilitate superior performance of multi-color flow cytometry assays. 

Features

Advanced optical design and sensitivity for multicolor assays

With customized forward scatter for the most accurate size measurement and optimized electronics for fast, accurate processing, the Gallios is designed to improve your cytometric workflow.

Improve your cytometric workflow and minimize downtime with remote diagnostics

​Choose from four scalable configurations to accommodate more lasers and fluorescence detectors as your multi-color flow cytometry research evolves.

  • Up-to four highly reliable, solid-state lasers in standard red and blue with optional violet and yellow lasers for greater choice of Fluorochromes to perform sophisticated multicolor assays
  • New 561nm laser capability for simultaneous analysis of multiple fluorescent proteins and greater detection functionality
  • Interchangeable optical filters to detect various dyes and wavelengths
  • Forward-scatter detector provides up-to three cell size and particle visualization measurements (to 0.404 µm in diameter)
  • Side-scatter detection via a dedicated high-performance photodiode with electronic attenuation
  • Six fluorescence detectors simultaneously acquire up-to six fluorescence signals (add as many as four additional detectors for concurrent reading of up-to 10 colors)
  • Simultaneous measurements of integral, peak and width available for all parameters, including scatter and fluorescence signals
Optics in action

The Gallios offers up-to four solid state, independently-focused diode lasers with an innovative steering design mechanism for optimal excitation at the flow cell yielding reliable high-quality results.

Emitted energy powered fiber optics flows to the boulevard via 18-degree reflective optics for enhanced light-collection efficiency and data resolution. The patented boulevard design offers maximum application flexibility with accessible plug-and-play filter and tandem dye collection features.

Electronics explained

The Gallios maintains a high yield of processed signals, greater than 90% even at 25,000 events/second.

  • 20 bit resolution
  • 1,048,576 channel display
  • More channels of resolution to separate even the dimmest of populations
  • Excellent accuracy and resolution for dim stains and quantitation

Patented electronics design ensures fast, accurate measurement of signals over a wide range of events.

  • Higher bits of information
  • Baseline restoration
  • Fast signal processing
  • Minimize data loss

High-yield electronics with fast pulse recovery at high speeds, the Gallios electronic system minimizes linearity errors and adjusts for temperature variability.

  • Sensitivity of dim particles
  • Minimize dead time

The Gallios electronic system has a self-correcting temperature regulation system.

  • Adjusts for temperature variability
  • Minimizes linearity errors based on temperature variations
Gallios System Specifications
Laser 6 colors/2 lasers (5 + 1 configuration)
Flow Product Line Gallios
Flow Product Type Instruments
Regulatory Status RUO

Training Courses

File/Info Title Ref. Number
Excitation Emission & Filters
Filter Selection
Flow Applications
Flow Compensation
Flow Cytometer
Flow Principles
Register for Onsite Training (Gallios)

Videos

  • Gallios - Electronics Explained

    Click to play  

    Optimized for high resolution and fast, accurate processing sensitivity and resolution are accomplished with excellent digital electronics design.

    The Gallios maintains a high yield of processed signals, greater than 90% even at 25k...

  • Gallios - Optics in Action

    Click to play  

    The Gallios offers up to four solid state diode lasers. The lasers are independently focused in the auto-beam tower using an innovative steering design mechanism that provides optimal excitation at the flow cell for reliable high quality...

  • Webinars & Symposiums

  • Gallios Forward Angle Light Scatter Innovation

    Bill Kirouac, Beckman Coulter

    Click to play  

    In flow cytometry, the FALS (Forward Angle Light Scatter) detector collects scattered light from a particle that intersects with a laser and delivers information roughly proportional to the size of the particle. Few innovations have occurred in...
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