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Instrumentation

Beckman Coulter offers a wide range of innovative cell sorters and analyzers to expand your clinical and research flow cytometry capabilities.

CytoFLEX S Flow Cytometer

Benchtop Cytometry without Compromises for Special Applications

The CytoFLEX S Flow Cytometer series is an expansion of the CytoFLEX Platform. The CytoFLEX Flow Cytometer offers three laser options, 488 nm, 638 nm, and 405 nm. The CytoFLEX S Flow Cytometer platform expands your research possibilities, with a fourth laser option, multiple configurations and unique filter sets for your science. CytoFLEX S Systems are available in preset configurations with up to four lasers, including 561 nm, 375 nm or lasers in the original palette and up to 13 channels for fluorescence detection.

Just like the CytoFLEX, the CytoFLEX S utilizes the same advanced technology to deliver superior sensitivity and resolution, comparable to or even exceeding instruments four times its size. The CytoFLEX Platform has a unique flow cell design and integrated optics. The innovative Wavelength Division Multiplexing (WDM) detection module includes high efficiency, low-noise Avalanche Photo Diode detectors for excellent performance. The revolutionary system presents optimal excitation and emission, minimizing light loss and maximizing sensitivity. Innovative technology enables the detection of dim populations with a software interface that is easy to use.

The CytoFLEX S is light (less than 22 kg) and compact (H 40.6 cm by W 40.5 cm by D 33 cm). You can install the CytoFLEX S where you need it on the benchtop or inside a standard biological safety cabinet.

Features

561 NM Laser

The addition of a 561 nm laser to the flow cytometer palette enables additional multicolor assays and allows you to move imaging assays to a flow cytometry format for easy quantitation. In addition, the 561 nm laser is a much more efficient method for exciting red fluorescent proteins, which excite poorly with a 488 nm laser. Use of a 561 nm laser increases the dynamic range and sensitivity you can achieve using these reporter proteins.

Optimal Detection of Fluorescent Proteins

The use of fluorescent protein reporter constructs has enabled a wide array of analyses including gene regulation, localization, and protein-protein interactions. Fruit fluorescent proteins provide added breadth to experiments however excite poorly with a 488 nm laser. Using the 561 nm laser of the CytoFLEX S Flow Cytometer allows you to see the fluorescent proteins, including the fruit dyes, you want.

Resolving Power for Dim Population Analysis

The complexity of heterogeneous cell population analysis continues to increase as more markers are needed to differentiate functional cell sub populations. With up to 13 channels for fluorescent detection, the CytoFLEX S Flow Cytometer has the capabilities needed to meet increased immunophenotyping demands for detecting rare cell types and dim populations.

375 NM Laser

CytoFLEX S 375 nm laser is a new addition to the CytoFLEX family of benchtop flow cytometers. The addition of the 375 nm laser, in a spatially separated discrete beam spot, enables excellent excitation of Hoechst, DAPI and brilliant UV dyes. This allows for experimentation with these dyes/fluorochromes without incurring the cost of a true UV laser.

Analysis Using Hoechst and DAPI

DNA intercalating dyes such as Hoechst and DAPI, which are both excited by the near UV laser, can both be used for cell cycle analysis. Hoechst dye is used to characterize “side population” through the elimination by ABC transporters; stem cells and early progenitor cells will eliminate Hoechst, while more mature cells will not, thus can be identified by low Hoechst fluorescence. Using the 375 nm laser, the CytoFLEX S Flow Cytometer can easily detect these populations.

Analysis of Brilliant UV Dye Excitation by Near UV Laser

Brilliant UV fluorochromes (BUV) can be utilized when designing high complexity cocktails and give additional flexibility in panel design.

Specifications

OPTICS

Excitation Optics

The CytoFLEX system can be configured with up to four spatially-separated lasers. The optical system is alignment free. The laser delays are automatically adjusted by the daily QC system. No user intervention is required to ensure optimum system performance.

Laser Specifications
Blue Laser Wavelength: 488 nm; Power: 50 mW; Beam spot size: 5 μm x 80 μm
Red Laser Wavelength: 638 nm; Power: 50 mW; Beam spot size: 5 μm x 80 μm
Violet Laser Wavelength: 405 nm; Power: 80 mW; Beam spot size: 5 μm x 80 μm
Yellow Laser Wavelength: 561 nm; Power: 30 mW; Beam spot size: 5 μm x 80 μm
Near UV Laser Wavelength: 375 nm; Power: 60 mW; Beam spot size: 5 μm x 80 μm
Flow Cell

The system does not require alignment. Fixed integrated optics and quartz flow cell design with >1.3 numerical aperture.

Flow Cell dimensions: 420 μm x 180 μm internal diameter

Forward Scatter Detection

Proprietary Axial Light Loss (ALL) sensor system using silicon photodiodes with built in 488/8 nm band pass filter.

Fluorescence and Side Scatter Detection

Fluorescence and side scatter light delivered by fiber optics to Avalanche Photo Diode detector arrays. Proprietary design ensures high performance, high efficiency, low-noise signal detection. Emission profiles are collected using reflective optics and single transmission band pass filters.

Violet Side Scatter Configuration

Option to configure Avalanche Photo Diode detector array to collect side scatter signal from Violet (405 nm) laser. The configured channel (VSSC) can be utilized to better resolve nanoparticles below 300 nm size threshold.

PERFORMANCE

 

Side Scatter Resolution

 

<300 nm

 

Violet Side Scatter Resolution (VSSC)

 

<200 nm

 

 
Forward and Side Scatter Resolution
Scatter performance is optimized for resolving human lymphocytes, monocytes, and granulocytes as well as nanoparticles.
 
Carryover
Single Tube Format: < 1.0%
Plate Loader Format: < 0.5%
 
Sensitivity
FITC: <30 molecules of equivalent soluble fluorochrome (MESF-FITC)
PE: <10 molecules of equivalent soluble fluorochrome (MESF-PE)
 
Fluorescence Resolution
The CytoFLEX S Flow Cytometer is capable of achieving 3% rCV with alignment verification particles capable of rCVs <3%.
 

QUALITY CONTROL

For detection channel off of the 405, 488, 561, and 638 nm laser, CytExpert QC automation pass/fail criteria is rCV <5.0%. For detection channels off of the 375 nm laser, the criteria is < 7.0%.
 

ELECTRONICS

Nominal Acquisition Rate
30,000 events per second with 15 parameters
Software capability to modify window extension parameter, to control abort rate during high event rate signal processing
 
Signal Processing
Fully digital system with 7 decade data display
 
Signal
Pulse area, height for every channel, width for one selectable channel
 

FLUIDICS

Ultra-low Pressure Peristaltic Sheath and Sample Delivery System
Low maintenance systen Sheath Pump tubing and Sample Pump tubing can be replaced by the user
(no service visit required)
 
Sample Flow Rates
Fixed Flow Rates: 10, 30 and 60 μL/min
Custom Flow Rate Control mode from 10 to 240 μL/min in 1 μL increments.
Gravimetric calibration.
Absolute count by volume, after simple calibration, using CytExpert software.
 
Fluid Capacity
Standard 4 L tanks
 
Automated Maintenance Cycles
Startup (initialize), sample mix, backflush, prime, shutdown (daily clean),
deep clean
 
Sample Input Formats
5 mL (12 x 75 mm) polystyrene and polypropylene
1.5 mL and 2 mL microcentrifuge
 
Plate Loader Formats
96-well Standard Flat, U and V Bottom plates
 

DATA MANAGEMENT

Software
CytExpert Software, fully featured proprietary application with exportable file formats for offline analysis, if desired.
Export FCS files feature allows data files to be read using Kaluza, FCSExpress, FlowJo and other software platforms
 
Language
English and Chinese
 
Operating System
Windows® 7 Professional 64-bit
 
FCS Format
FCS 3.0
 
Minimum Specifications
CPU: Intel® I3 @ 2.9 GHz 1 Gigabit Ethernet port
RAM: 4 GB 2 USB 3.0 ports
Storage: 256 GB 4 USB 2.0 ports
 
Compensation
Automatic Full matrix compensation
Manual full matrix compensation
Novel Compensation Library for storage of spillover values of dyes to easily determine the correct compensation matrix with new gain settings
Ability to import/export compensation values between experiments, using compensation library function
Absolute linear gain amplification, enabling the use of compensation settings between experiments and sample types
 

INSTALLATION

Dimensions (W x D x H)
Cytometer (with or without Plate Loader) Tanks and Holder
42.5 cm x 42.5 cm x 34 cm 14 cm x 35.6 cm x 35.6 cm
16.7 in x 16.7 in x 13.4 in 5.5 in x 14.0 in x 14.0 in
 
Weight

Cytometer: 23.4 kg / 51.6 lbs

Cytometer with Plate Loader: 28kgs / 61.7 lbs

Power Specifications

Voltage: 100–240 V

Power: 150 -250 W

Operating Temperature Non-Condensing

15-27 °C, 59-80.6 °F

CONFIGURATIONS

Preset configurations available with up to four lasers and up to 13 channels for fluorescence detection.*

Commonly used Fluorescent Dyes Laser Fluorescent Channel Part number
B78558 B78561 B78559 B78560 B78557 B75812 B75811 B75408
Number of Detectors 6 8 10 12 13 6 9 13
DAPI, Hoechst Blue 375 nm 450/45 BP      
Hoechst Red 675/30 BP      
Pacific Blue dye, V450, eFluor450, BV421 405 nm 450/45 BP          
Krome Orange, AmCyan, V500, BV510 525/40 BP          
Bv605, Qdot605 610/20 BP          
BV650, Qdot655 660/20 BP            
FITC, Alexa Flour488, CFSE, Fluo-3 488 nm 525/40 BP
PE, PI 585/42 BP          
ECD, PE-Texas Red, PE-CF594, PI 610/20 BP            
PC5.5, PC5, PerCP, PerCP-Cy5.5, PI 690/50 BP
PC7 780/60 BP          
PE, PI 561 nm 585/42 BP      
ECD, PE-Texas Red, PE-CF594, PI 610/20 BP      
PC5.5, PC5, PerCP, PerCP-Cy5.5, PI 690/50 BP      
PC7 780/60 BP      
APC, Alexa Fluor 647, eFluor 660 638 nm 660/20 BP        
APC-A700, Alexa Fluor 700 712/25 BP        
APC-A750, APC Cy7, APC-H7, APC eFluor 780 780/60 BP        

*Additional custom filter sets are also available for user installation. Ask your sales representative for details.

Accessories

Use Less Sample and Maximize Data Collected

Use sample volumes as low as 10 μL. Detect up to 30,000 events per second with 15 parameters. Pick a pre-set flow rate of 10, 30, or 60 μL/min or use the custom setting to adjust over the full range from 10 to 240 μL/min. For higher throughput applications an optional plate loader module can save hands on time without expanding the instrument footprint.
 
Highlighted Features:
  • Plate Loader option can analyze a 96-well plate in as little as 32 minutes
  • Switch between single tube and plate acquisition in 5 minutes 
  • Easy virtual plate layout setup with customizable wash and mix cycles
  • Define multiple experiments on a single plate
  • Compatible with flat-bottom, U- and V-bottom standard 96-well plates

Videos

  • CytoFLEX: The Magic Inside

    Click to play  

    CytoFLEX Flow Cytometer is the first commercially available system to utilize compact APDs and reducing overall instrument footprint.
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