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Instrumentation

Beckman Coulter offers a wide range of innovative cell sorters and analyzers to expand your clinical and research flow cytometry capabilities.

CytoFLEX Flow Cytometer

Ultraflexible performance and resolution

The CytoFLEX system brings you easily upgradeable detection capabilities for up to 3 lasers and 13 color research flow cytometry right on your bench top. Providing quality and performance at any configuration, the CytoFLEX system provides powerful sensitivity and resolution for the simple to the most challenging applications. CytoFLEX delivers and surpasses capabilities expected in top tier analyzers, with excellent performance and nanoparticle resolution.

 

Use this interactive quoter tool to select the configuration that fits your research needs.


Features

Quality performance, multiple configurations for real power on your benchtop

The high-performance CytoFLEX system offers scalable detection capabilities for up-to three lasers and 13-color research flow cytometry right on your benchtop.

Ready to run in minutes, not hours with CytoFLEX

The CytoFLEX system is easy to learn, easy to operate and easy to maintain.

cytoflex flow cytometer
  • Superior sensitivity and resolution for excellent fluorescence and nanoparticle detection
  • Compact, reliable design fits right on your benchtop (<50lbs, 16”x16”x13”)
  • Future-proof system with easy upgrade options
  • Powerful performance with up-to 21 flexible configurations (up-to three Lasers, 13 colors)
  • CytExpert experiment-based software for easy multicolor setup and QC
  • Versatile plug-n-play filters (bandpass-only light collection)
  • Integrated optics for alignment stability
  • Accommodates 12x75mm and microtiter tubes

Sensitivity

An intelligent patent-pending optical design optimizes excitation from up-to three lasers (488nm; 638nm; 405nm) and light collection efficiency. With unique flow cell design and integrated optics, the innovative Wavelength Division Multiplexing (WDM) detection module includes solid-state, high efficiency, low-noise detectors for excellent performance. The WDM’s bandpass-only design guarantees flexibility with easily changeable filters and 4-13 configurations.

Nano particle detection

When equipped with a violet laser, the CytoFLEX can be set up to measure Side Scatter from 405nm for enhanced nanoparticle detection. Mie theory predicts that the scattering cross section of a particle, and thus its scattering intensity, depends on the wavelength of light, the angle of collection, and the size, shape, and refractive index of the particle. All other factors being equal, using a shorter illumination wavelength will result in an increase in scattering cross section, and thus more scattered light. The CytoFLEX offers light scattering parameter measurements from the 488nm laser light, and optionally from the 405nm laser to enhance small particle detection.

Maximize investment in CytoFLEX with custom configuration options

With future-proof upgrade options for up-to 13 colors, the CytoFLEX system evolves as your application needs change. Upgrade your configuration in less than one day knowing that all CytoFLEX instruments have been fully tested across all configuration types.

Excitation Fluorescence
Channels
Fluorochromes
488nm 525/40 BP FITC, Alexa Fluor 488, CFSE, Fluo-3
585/42 BP PE, PI
610/20 BP ECD, PE-Texas Red, PE-CF594, PI
690/50 BP PC5, PC5.5, PerCP, PerCP-Cy5.5, PI
780/60 BP PC7
638nm 660/20 BP APC, Alex Fluor 647, eFluor 660
712/25 BP APC-Alexa Fluor 700, Alexa Fluor 700
780/60 BP APC-Alexa Fluor 750, APC-Cy7, APC-H7, APC-eFlour 780
405nm 450/45 BP Pacific Blue, V450, eFluor 450, BV421
525/40 BP Krome Orange, AmCyan, V500, BV510
610/20 BP Violet610, BV605, Qdot 605
660/20 BP Violet660, BV650, Qdot 655
780/60 BP Viole780, BV785, Qdot 800

Training Courses

File/Info Title Ref. Number
CytoFLEX Online Operator Training

Videos

  • CytoFLEX: It Fits

    Click to play  

  • CytoFLEX: Powerful, Flexible, Easy

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  • CytoFLEX: Special Procedure

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  • CytoFLEX: The Magic Inside

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    CytoFLEX Flow Cytometer is the first commercially available system to utilize compact APDs and reducing overall instrument footprint.
  • Installing Your New CytoFLEX Plate Loader

    Click to play  

  • Unboxing Your New CytoFLEX Flow Cytometer

    Click to play  

  • Products

    Flow Cytometry Instruments & Software - Clinical Diagnostics

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    B51503

    CytoFLEX Sheath Fluid, 1 x 10 L

    RUO

    $27.00

    B53017

    CytoFLEX System B2-R0-V2

    488nm (50mW) + 405nm (80mW)

    4 Fluorescent Detectors

    RUO

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    B53016

    CytoFLEX System B2-R2-V0

    488nm (50mW) + 638nm (50mW)

    4 Fluorescent Detectors

    RUO

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    B53012

    CytoFLEX System B3-R0-V3

    488nm (50mW) + 638nm (50mW) + 405nm (80mW)

    6 Fluorescent Detectors

    RUO

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    B53015

    CytoFLEX System B3-R1-V0

    488nm (50mW) + 638nm (50mW)

    4 Fluorescent Detectors

    RUO

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    B53014

    CytoFLEX System B3-R2-V0

    488nm (50mW) + 638nm (50mW)

    5 Fluorescent Detectors

    RUO

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    B53010

    CytoFLEX System B3-R2-V2

    488nm (50mW) + 638nm (50mW) + 405nm (80mW)

    7 Fluorescent Detectors

    RUO

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    B53011

    CytoFLEX System B3-R3-V0

    488nm (50mW) + 638nm (50mW) + 405nm (80mW)

    6 Fluorescent Detectors

    RUO

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    B53009

    CytoFLEX System B3-R3-V2

    488nm (50mW) + 638nm (50mW) + 405nm (80mW)

    8 Fluorescent Detectors

    RUO

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    B53007

    CytoFLEX System B3-R3-V3

    488nm (50mW) + 638nm (50mW) + 405nm (80mW)

    9 Fluorescent Detectors

    RUO

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    B53004

    CytoFLEX System B3-R3-V5

    488nm (50mW) + 638nm (50mW) + 405nm (80mW)

    11 Fluorescent Detectors

    RUO

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    B53019

    CytoFLEX System B4-R0-V0

    488nm (50mW)

    4 Fluorescent Detectors

    RUO

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    B53013

    CytoFLEX System B4-R2-V0

    488nm (50mW) + 638nm (50mW)

    6 Fluorescent Detectors

    RUO

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    B53008

    CytoFLEX System B4-R3-V2

    488nm (50mW) + 638nm (50mW) + 405nm (80mW)

    9 Fluorescent Detectors

    RUO

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    B53006

    CytoFLEX System B4-R3-V3

    488nm (50mW) + 638nm (50mW) + 405nm (80mW)

    10 Fluorescent Detectors

    RUO

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    B53003

    CytoFLEX System B4-R3-V4

    488nm (50mW) + 638nm (50mW) + 405nm (80mW)

    13 Fluorescent Detectors

    RUO

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    B53002

    CytoFLEX System B4-R3-V5

    488nm (50mW) + 638nm (50mW) + 405nm (80mW)

    12 Fluorescent Detectors

    RUO

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    B53018

    CytoFLEX System B5-R0-V0

    488nm (50mW)

    5 Fluorescent Detectors

    RUO

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    B53037

    CytoFLEX System B5-R0-V5

    RUO

    Request Quote

    B53005

    CytoFLEX System B5-R3-V2

    488nm (50mW) + 638nm (50mW) + 405nm (80mW)

    10 Fluorescent Detectors

    RUO

    Request Quote

    B53001

    CytoFLEX System B5-R3-V4

    488nm (50mW) + 638nm (50mW) + 405nm (80mW)

    12 Fluorescent Detectors

    RUO

    Request Quote

    B53000

    CytoFLEX System B5-R3-V5

    488nm (50mW) + 638nm (50mW) + 405nm (80mW)

    13 Fluorescent Detectors

    RUO

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    For Research Use Only. Not for use in diagnostic or therapeutic procedures.
    Class 1 Laser Product
    CytoFLEX will be available in selected geographies. Please check with your Beckman Coulter representative, for availability.
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