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Instrumentation

Beckman Coulter offers a wide range of innovative cell sorters and analyzers to expand your clinical and research flow cytometry capabilities.

MoFlo Astrios EQ

Complex sorting, simplified

Answer tomorrow’s research questions today with the MoFlo® Astrios™ EQ. Perform 6-way sorting without compromise, enhance operator safety, and let your application determine your instrument parameters, not the other way around.

The MoFlo Astrios EQ was designed around the needs of today’s research scientists — broader analytical capability, robust biohazard containment, application flexibility, and consistently trustworthy data. The MoFlo Astrios EQ is easy, intuitive, and flexible — and lets you see what other sorters cannot.

With the MoFlo Astrios EQ, you never have to be limited by your instrumentation. Its patent pending dual enhanced forward scatter (eFSC) technology enables you to see and sort particles you might otherwise miss. Measure crucial particle characteristics over more than three orders of magnitude, from as small as 200 nm to 30 µm diameter, all at the same time. You can also trust MoFlo’s proven fluidics to be gentle with your biological material.

Fluorochrome Guide

Enhanced Forward Scatter

Removing your constraints
Our latest development, eFSC (enhanced Forward Scatter), permits simultaneous detection and sorting of a wide range of particle sizes yet, and offers the potential to open new insights into your experimental milieu.

Our fastest and most precise sort deposition technology, coupled with mixed-mode sorting, will allow you to move into assay miniaturization to make more of your precious samples.

Multiple available levels of biohazard control enable you to work with potentially biohazardous materials that you would previously have only been able to analyze, not sort.

Beckman Coulter is committed to continuing the path of innovation allowing you to do more with your MoFlo Astrios EQ than you dreamed was possible yesterday.

Bringing new data within reach

Go beyond conventional sorters and expand your research horizons with the MoFlo Astrios EQ.

  • Broader analytical capability
  • Robust biohazard containment
  • Application flexibility
  • Consistently trustworthy data

MoFlo Users Group (MUG)


For users of Beckman Coulter's MoFlo instrument to discuss applications and exchange ideas.


Accelerate cell sorting with the first true 32-bit high-resolution 5-decade multi-channel system

Simultaneously measure crucial particle characteristics in more than three orders of magnitude.

  • 7 lasers, 2 alignment stages
  • Resolve particles (beads and cells) down to 0.2 µm
  • Sort rare event populations with 99% purity
  • Flexible experiment design with 6-way mixed mode sorting
  • Forward scatter detection support for 6 laser paths
  • Optional side scatter off any laser (excluding UV)
  • Minimum triggering threshold reduced to 0.001% for enhanced resolution
  • Seven mask designs for differing applications
  • ABS ND filter set 0.3, 0.6, 1.0, 1.3, 2.0
  • Fluidic and air filtration enhancements:
    • Gentle with your biological material
    • Sheath filter 0.04 µm particles
    • Air filter 0.01µm particles
    • Unequalled resolving FSC & FL power through adjustable 9-decade dynamic range for greater certainty when resolving background from dim staining populations and/or broad dynamic range applications
MoFLO Astrios EQ Enhanced forward scatter

Apply application-specific masks for different particle types and use different neutral density filters for improved overall particle size range. The top histogram is measured on eFSC2 with no neutral density (ND) filter to widen the separation between noise and 0.2 and 0.3 μm beads. The second and third histograms display eFSC1 data (acquired with an ND1 filter) with different y-axis scales. The entire dynamic range from 0.2 - 30 μm is easily visible when different ND filters are used on each eFSC parameter.

Performance

In conventional cell sorters, there is a three-way trade-off between sort speed, sort purity and sort yield. The MoFlo Astrios EQ Mixed Mode sorting allows selecting for purity in one sort-direction and yield in another, enabling simultaneous recovery of virtually all the target cells that would otherwise be lost to achieve that high purity. This enables you to sort faster without losing extremely valuable cells, and without sacrificing purity of the principal sort.

Perform 6-way sorting without compromise
  • Save time by selecting more populations at once
  • Save precious sample with our unique mixed-mode sorting capability
Break free from cuvette sorting restrictions
  • Analyze heterogeneous populations with confidence — no differential velocities based on particle size; eliminate the need for Area:Height scaling and laser-delay optimization
  • Maintain consistent performance with less maintenance — no concern about burn-in of debris on cuvette
  • Allow biological demands to dictate sorting conditions — select from 70 and 100 micron nozzle sizes, to precisely accommodate cells from practically any tissue you want using Intellisort II to automatically maintain drop delay
Enjoy unsurpassed plating efficiency
  • Finest deposition accuracy
  • Achieve high throughput single-cell deposition rates for an entire 96-well plate within 75 seconds and an accuracy of 99 % from a 1 % sort population
  • Broad plate range available (6 to 1536 well plates)
  • Achieve better precision with uncharged drop collection where required
Analyze and sort from microsomes to macrophages, from astrocytes to xenografts
  • Work with an extensive range of biological and non-biological particles
  • Consider non-biological applications like agarose gel droplets, TiO2 and gold nanoparticles, or environmental particulates
Fine-tune the instrument to suit your application
  • Match nozzle size to most experimental demands
  • See large and small events together with optimized forward scatter masks
  • Gain greater freedom to adjust pressures to handle wide range of cell types from multiple tissue sources
  • Enhance cell viability and throughput with sample agitation and temperature control

Optics

Accelerate cell sorting with the first true 32-bit high-resolution 5-decade multi-channel system

Excitation Optics—Laser Palette

  • Seven discrete laser interrogation points
  • Seven pinholes
Simultaneously measure crucial particle characteristics in more than three orders of magnitude.
Wavelength Laser Power Elliptical shape
355nm 100 mW Flat-Top shape
405nm 55 mW Flat-Top shape
488nm 200 mW Flat-Top shape
532nm 150 mW Flat-Top shape
561nm 200 mW Flat-Top shape
592nm 200 mW Flat-Top shape
642nm 100 mW Flat-Top shape
Standard Filter Sets Optimized per Laser Wavelength
355nm PI, Hoechst (red and blue), DAPI
405nm SSC, Cascade Blue*, Pacific Blue*, Cascade Yellow*
488nm SSC, FITC, PE, ECD (PE-Texas Red*), PE-Cy5/PerCP, PE-Cy5.5, PE-Cy7
532nm SSC, PE, PE-Alexa* 610, PE-Alexa 647, PE-Cy5.5, PE-Alexa 700
561nm SSC, mCherry, DSRed, mPlum, HCRed
592nm SSC, Texas Red, APC, APC-Cy7, Alexa 647, Alexa 700, Alexa 750
642nm SSC, APC, APC-Cy7, Alexa 647, Alexa 700, Alexa 750
Emission Optics
Resolution Resolves minimum 0.2µm diameter particles in scatter from background.
Fluorescence Sensitivity Fluorescence sensitivity of < 125 MESF for FITC and <110 MESF for PE using Spherotech 8-peak beads using 488nm laser.
Optical Parameters Up-to 44, including two FSC
Fluorescence half height CV precision FITC<2.4%, PE <2.4%, PE-TR <2.6%, PE-Cy5 <3.2%, PE-Cy7 <4.3%, APC <2.3%, APC-Cy7 <2.8% (1x 106 SPHERO™ Ultra Rainbow beads at sample flow rate of 6µl/min, 100-120 EPS)

Biological Safety

Maximize safety without compromising workflow

Designed from the start with biosafety foremost in mind, the MoFlo Astrios EQ has a sort chamber that features rounded corners and edges throughout to facilitate cleaning. It also includes multiple levels of interlocks to protect users during normal operation from laser, electrical and biohazard risks.

The optional Baker Company SterilGARD biological safety cabinet can be fully integrated with the MoFlo Astrios EQ, to provide assured biological safety level 2 (BSL2) containment without compromising workflow, thanks to the aerodynamic design of the MoFlo Astrios EQ itself. The BSL 2 cabinet is fully tested to NSF-49 international standards when enclosing the MoFlo Astrios EQ. The r-theta carrier arm mechanism helps eliminate cross-contamination between sorts by maintaining a sealed and easily cleaned sort chamber. The aerosol evacuation system pulls particulates from three chambers with verified performance to meet ISAC guidelines.

  • Built-in Aerosol Evacuation Port that accommodates the MoFlo Astrios EQ’s unique three-aerosol evacuation chambers – no need for additional aerosol management options (unless required by specific lab operating procedures) or external building exhaust infrastructure.
  • Fully sealed sort chamber accessible via an R-Theta CyClone® arm that ensures aerosols are captured and evacuated via SterilGARD HEPA filtration system.
  • Cabinet-2-Sorter Interlock controller between the MoFlo Astrios EQ and the Baker SterilGARD cabinet ensures biosafety is maintained at all times during aerosol generation.
  • Zero hard edges to simplify surface cleaning and maintain aseptic sort environment.
  • Floor-standing design, which includes the MoFlo Astrios EQ lower module and accommodates most standard laboratory spaces.

Sample and Sorting Applications

IntelliSort II Beadless Drop Delay

Minimize your risk of sample contamination and maintain integrity of sorted samples with uninterrupted aseptic sorting.

  • No fluorescent beads or microscope required
  • Camera images and proprietary algorithms allow you to determine optimal drop delay without using fluorescent particles in the sample line
  • Maintain sterile samples during drop-delay recalculation with uninterrupted sterility
  • Maintain drop-delay functionality with 50µm and 150µm nozzle tips
  • Automatic QC parameters
    • Sheath and sample pressure
    • Average event rate
    • Start and end times
    • Nozzle size
    • Differential between measured parameters and pass/fail thresholds
    • QC data exclusion
  • Laser-delay parameters
    • Date of last laser delay calculation
    • Trigger channel
    • Sheath pressure and nozzle size
    • Powered laser wavelengths
    • Calculated laser delays automatically displayed

You determine your application parameters not your instrument
Work with the greatest possible range of biological and non-biological particles knowing you can fine-tune your MoFlo Astrios EQ to suit your specific application. Adjust pressures to accommodate a wide range of cell types. Improve cell vitality without compromising sort speed and keep your cells alive and running with temperature and agitation options. 

Run a wide range of single-cell applications, including:

  • Circulating tumor cells (CTCs)
  • Drug discovery applications
  • Rare event analysis
  • Stem cells
  • Neuronal cells
  • Fluorescent protein reporter selection
  • Ecological and physiological microbes (yeasts and bacteria)

Software

Faster setup and advanced functionality with Summit Software

Summit v6.2 Software

  • Support for multiple forward scatter parameters in Summit and FCS file format
  • Two additional PMT channels for forward scatter collection
  • Improved usable dynamic range with PMT voltage of 100 volts
  • Enhanced functionality and support for user-defined filters and fluorochromes
  • Automated quality control functionality

Innovation and Development

Beckman Coulter today is the inheritor of the pioneers of modern cell analysis. We continue that proud tradition of innovation with complete cytometry solutions. The MoFlo Astrios EQ is an instrument you can depend upon, from a company you know and trust.

Beginning with pH meters designed by Arnold Beckman and commercialized from 1935 and automated particle counters based upon the Coulter Principle invented by Wallace Coulter and patented in 1953, Beckman Coulter has a long history of innovation and development in the science of measurement, particle characterization, and sorting.

Flow cytometry has been part of our core business since the first EPICS II cell sorter in 1974. It is a proud tradition and one that you can be sure the company will continue to support and develop, with complete cytometry solutions encompassing sample preparation, reagents, detection and analysis software.

The acquisition of the extremely successful MoFlo high-speed cell sorter platform and the on-going development of this technology attests further to this commitment to the future, and to the support of scientists pushing the boundaries of cell biology research.

Training Courses

File/Info Title Ref. Number
Flow Applications
Register for Onsite Training (MoFlo Astrios)
The Art of Sorting

Videos

  • MoFlo Astrios - The Evolution Continues

    Click to play  

    Researchers rely on Beckman Coulter's historic cell sorter technology. Now the next generation is here. The MoFlo Astrios is the new standard for cell sorting with functionality, speed, and improved sensitivity. It is designed for researchers...
  • Webinars & Symposiums

  • DuraClone - A New Frontier in Dry Reagents

    Aprajita Priyadarshini, Sr. Applications Scientist, Beckman Coulter

    Click to play  

    DuraClone is an innovative approach for dry reagents for flow cytometry. The proprietary process results in a product that is consistent from tube to tube and lot to lot and eliminates the need for cold chain storage. The process is distinct...

  • Extracellular Vesicle Isolation by Flow Cytometric Sorting and Characterization by Analytical Ultra-Centrifugation and Dynamic Light Scatter

    Carley Ross, PhD Staff Development Scientist, Beckman Coulter

    Click to play  

    The extracellular vesicle (EV) research field has dramatically increased in the last five years. Using a high-speed flow cytometric sorter, EVs may be isolated at high rates such that researchers can differentially separate, isolate and...
  • Hematopoletic Bone Marrow Cell Sorting Without Compromise: Seven Lasers, Eight Colors, Six-Way Sorting - A Novel Approach

    John Tigges Technical Director, Flow Cytometry Core Beth Israel Deaconess Medical Center, Boston, MA

    Click to play  

    Flow cytometry analysis and sorting are valuable techniques for studying the distinct hematopoietic bone marrow populations. Identification, isolation and purification of hematopoietic stem cells (Long Term and Short Term) and progenitors (Common...
  • Microvesicle Detection and Cell Sorting

    Vasilis Toxavidis, MChS, HCPR Resource Director, Beth Israel Deaconess Medical Center Flow Cytometry Core Facility, Harvard Stem Cell Institute John Tigges, Beth Israel Deaconess Medical Center Flow

    Click to play  

    There is great interest in both medical and scientific communities in submicron cell derived particles, termed microparticles or microvesicles. A great difficulty in this field, however, has been the optimization and standardization of...

  • Stem Cells: Identification & Isolation through High-Speed Cell Sorting

    E. Michael Meyer, Research Scientist Manager, Flow Cytometry Facility University of Pittsburgh Cancer Institute

    Click to play  

    The discussion of Stem Cells can cover a range of topics and cell types; hematopoietic progenitor cells, mesenchymal, adipose, and even "cancer" stem cells are just few of the types of stem cells that we work with on a daily basis...

  • The Art of Sorting

    David P. Mcintyre Senior Specialist Technical Training Beckman Coulter, Inc., Miami, FL

    Click to play  

    This presentation will illustrate the basic elements that are needed to sort successfully. These include: droplet breakoff, particle detection, sort decisions, sort delay, droplet charging and droplet deflection. Each element will be discussed in...
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    For research use only. Not for use in diagnostic procedures. Class I laser product.
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