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DRAQ7 Dye

DRAQ7 Dye

DRAQ7™ is a new far-red fluorescent DNA dye that ONLY stains the nuclei in dead and permeabilized cells. It can be combined with other common fluors, especially GFP fusions & FITC and PE labeled antibodies.

It can be excited by the blue, green or red light in a flow cytometer and red light in imaging instruments.

  • Does not enter intact, live cells. It is excited by blue through red lasers.
  • Non-toxic in long-term culture
  • Ideal replacement for propidium iodide (and 7-AAD) having far better spectral properties: no UV excitation and no emission overlap with PE and homologues.
  • Allows a new design of combination analysis for cell health and status.

Features

DRAQ7 has many applications and is highly compatible with existing protocols across a wide range of instrumentation platforms.

  • Rapid staining of dsDNA/nuclei of dead or permeabilized cells
  • Combination with live cell dyes for dead/live discrimination
  • Easy to use - no wash, no RNase needed
  • Ideal for use with GFP & FITC labels - DRAQ7 fluoresces in the far-red region
  • DRAQ7 is an ideal far-red DNA counterstain for IF/IHC, high content screening and cell-based assays

Spectral Features:

  • Fluorescence emission from 665 nm into the low infra-red
  • Suggested optimal emission filters include 695LP, 715LP or 780 LP
  • Optically compatible with benchtop flow & laser scanning cytometers and epifluorescent & non-UV confocal microscopes
  • Useful excitation at sub-optimal wavelengths from the 488 nm, 568 nm and 633 nm lines, plus optimal 647 nm excitation. No UV excitation, unlike propidium iodide.
  • No compensation needed with common FITC/GFP + PE combinations in flow cytometry. Can be combined with many live cell dyes.
  • Allows multi-line imaging/cytometry when combined with UV & vis. range fluorochromes
  • No apparent fluorescence enhancement upon binding to DNA
  • Photobleaching not normally observed

Spectral Profile:

The graphs below show standard absorbance and fluorescence emission spectra for DRAQ7 in aqueous solution for a range of different excitation wavelengths. As can be seen, DRAQ7 can be effectively excited by 488nm systems. In these circumstances, we recommend that a long band pass filter be used - to capture as many emitted photons as possible.

DRAQ7 Dye - Excitation

Excitation:

  • 633 & 647 nm line optimal (Exλmax 599/644 nm)
  • 488, 514 and 568 lines, sub-optimal (only by flow cytometry)
 
DRAQ7 Dye - Emission

Emission:

  • >665 nm to infra-red >800 nm (Emλmax 678 nm / 694 nm intercalated with dsDNA)
  • Minimal overlap with visible range e.g. GFP and FITC
  • Emission filters may include 695LP, 715LP, 725 BP 20 or 780 LP

DRAQ7 enters and labels only membrane-compromised cells.

The flow cytometric data below shows apoptosis (compromised membranes permitting entry of DRAQ7) of human Jurkat cells exposed to 0.1 - 2.0 µM staurosporine for 24h compared to negative controls.

DRAQ7 Dye - Fluorescence Intensity

DRAQ7 can be used in flow cytometric analysis to mark membrane-compromised cells, in combined with reagents such as Annexin V, mitochondrial membrane probes and other reporters of cell health. Live, intact cell impermeant DNA dyes are commonly combined with Annexin V (which reports membrane inversion) to give information about the progression of apoptosis. As shown in the figure below, DRAQ7 (top & middle series) performs in an equivalent manner to propidium iodide (PI, bottom series), to report the onset of membrane leakiness caused by increasing doses of VP-16. Plus, DRAQ7 offers new possibilities as a far-red, non-enhancing DNA binding dye. Using both log and linear displays, further discrete sub-populations can be identified. Also, unlike PI, DRAQ7 will permit several fluorescent parameters to be measured in parallel; 4 with blue laser excitation.

DRAQ7 Dye - Cell Viability

DRAQ7 can be used in apoptosis assays, cytotoxicity assays, cytolytic antibody testing, cell health assays.

Due to its far-red emission signal, DRAQ7 can be easily combined with multiple other fluorophores; up to 4 using the single 488 nm excitation source (argon-ion laser).

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B25595

DRAQ7™ Far-Red Fluorescent Live-Cell Impermeant DNA Dye

RUO

$280.00

For Research Use Only. Not for use in diagnostic procedures.
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