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Rotors

Get the most from your centrifuge system with Beckman Coulter rotors designed for maximum versatility and performance.

Our extensive rotor portfolio supports labware from microplates and microtubes to 1 Liter bottles for your evolving research needs.
 
Designed for diverse processing applications—

  • Bioprocessing
  • Cellular biology
  • Diagnostic and clinical biology
  • Genomics and molecular biology  
  • Protein research
  • Research and drug discovery 
  • Virus and nanotechnology research 

Analytical Ultracentrifuge

    • An-50 Ti Rotor

      An-50 Ti Rotor

      High-throughput analytical rotor capable of analyzing 7 samples in each run up to 50,000 rpm. Made of titanium, the AN-50 Ti analytical rotor has minimal temperature fluctuations, essential to analyzing particles by analytical ultracentrifugation (AUC). AUC is used to characterize size, shape, heterogeneity, molecular weight, stoichiometry, conjugation efficiency, payload, and binding rates of proteins, nucleic acids, nanoparticles, colloids, peptides, and viral vectors.

    • An-60 Ti Rotor

      An-60 Ti Rotor

      High-speed analytical rotor capable of analyzing 3 samples in each run up to 60,000 rpm. Made of titanium, the AN-50 Ti analytical rotor has minimal temperature fluctuations, essential to analyzing particles by analytical ultracentrifugation (AUC). AUC is used to characterize size, shape, heterogeneity, molecular weight, stoichiometry, conjugation efficiency, payload, and binding rates of proteins, nucleic acids, nanoparticles, colloids, peptides, and viral vectors.

High Performance

    • JA-10 Fixed Angle Rotor

      JA-10 Fixed Angle Rotor

      Classical rotor for 3L application. Consistent separation with 5 min approximate deceleration time from maximum speed (rotor fully loaded, max. brake).

    • JA-14 Fixed Angle Rotor

      JA-14 Fixed Angle Rotor

      Fixed-angle, aluminum rotor for general-purpose, large-volume and multi-tube processing.

    • JA-14.50 Fixed Angle Rotor

      JA-14.50 Fixed Angle Rotor

      Fixed-angle, aluminum rotor with a high force and large volume. Ideal for pelleting and density gradient separation of proteins, lysate/tissue homogenates, nucleic acids, cells, viruses and blood. Capable of spinning 50, 15, 10, 5 and 1.5 mL tubes at their maximum speeds.

    • JA-17 Fixed Angle Rotor

      JA-17 Fixed Angle Rotor

      Fixed-angle rotor made of aluminum, with a high force and large volume. Ideal for pelleting bacteria, cell membranes, and subcellular organelles.

    • JA-18 Fixed Angle Rotor

      JA-18 Fixed Angle Rotor

      High force, high volume, fixed angle aluminum rotor. Good for pelleting bacteria, cell membranes, and subcellular organelles. Includes 24 reversible adapters that provide 18,000 rpm maximum speed.

    • JA-18.1 Fixed Angle Rotor

      JA-18.1 Fixed Angle Rotor

      Aluminum fixed-angle rotor used for high-force sedimentation in microcentrifuge tubes under refrigerated conditions. Includes 24 reversible adapters that provide 18,000 rpm maximum speed.

    • JA-20 Fixed Angle Rotor

      JA-20 Fixed Angle Rotor

      Aluminum, fixed angle rotor for harvesting bacteria and cell membranes, processing tissue homogenates, and separating cell particulates.

    • JA-20.1 Fixed Angle Rotor

      JA-20.1 Fixed Angle Rotor

      Aluminum, fixed-angle rotor that’s high force and provides large-volume separation of particles with 100 S or larger sedimentation coefficients.

    • JA-21 Fixed Angle Rotor

      JA-21 Fixed Angle Rotor

      Aluminum, fixed angle rotor used for rapid, efficient separation of many samples in small volumes, such as viruses, bacteriophage, mitochondria, and nuclei.

    • JA-25.15 Fixed Angle Rotor

      JA-25.15 Fixed Angle Rotor

      Aluminum, fixed angle rotor used for harvesting bacteria, processing tissue homogenates, subcellular particulates, and routine pelleting such as precipitates and phase separations.

    • JA-25.50 Fixed Angle Rotor

      JA-25.50 Fixed Angle Rotor

      Fixed-angle, aluminum rotor, used for harvesting bacteria, processing tissue homogenates, subcellular particulates, and routine pelleting such as precipitates and phase separations.

    • JA-30.50 Ti Fixed Angle Rotor

      JA-30.50 Ti Fixed Angle Rotor

      Titanium, fixed-angle rotor, used for harvesting bacteria, processing tissue homogenates, subcellular particulates, and routine pelleting such as precipitates and phase separations.

    • JCF-Z Zonal and Continuous Flow

      Continuous flow processing in the JCF-Z rotor allows efficient processing (pelleting, purification, or concentration) of large sample volumes containing particles with 600 S or higher sedimentation coefficients. This rotor is suitable for sedimenting bacteria, yeast, large subcellular particles such as membranes, and mitochondria. It is also frequently used to clear virus-containing culture media of cellular debris before final isolation of the virus. In the JCF-Z rotor, the zonal technique is useful in separating large particles, such as mammalian viruses and subcellular organelles, from plant or animal tissue homogenates.

    • JCF-Z Zonal and Reorienting Gradient Rotor

      In density gradient separation, sample capacity is limited by the area in the sample cavity over which the sample spreads. With a zonal rotor, the gradient and sample are loaded directly into the rotor cavity. Zonal centrifugation enables larger quantity of sample to be processed in less time than in other types of rotors. This rotor is suitable for sedimenting tissue homogenate, small and large viruses, bacteria and whole cells.

    • JE-5.0 Elutriator Rotor

      JE-5.0 Elutriator Rotor

      Centrifugal elutriation in the JE-5.0 rotor combines two separation technologies: centrifugation, the process of sedimentation under the influence of a centrifugal force field, and counterflow elutriation, the process of separation by washing. Cells retain their viability with gentle washing and low g force. Layer separation without using harsh cushion/gradient chemicals and no multiple steps separation.

    • J-LITE JLA 10.5000 Fixed Angle Rotor

      J-LITE JLA 10.5000 Fixed Angle Rotor

      Lightweight, large-volume rotor made of anodized aluminum containing 6 carbon-fiber canisters with secondary liquid closures. This rotor is used for the initial processing of bacteria or other cells from fermentors, clearing cell debris from homogenates, or pelleting subcellular organelles and protein precipitates.

    • J-LITE JLA 9.1000 Fixed Angle Rotor

      J-LITE JLA 9.1000 Fixed Angle Rotor

      Large-volume, aluminum rotor with carbon-fiber canisters for large-volume processing, pelleting of bacteria, subcellular organelles, viruses and precipitates. 

    • J-LITE JLA 8.1000 Fixed Angle Rotor

      J-LITE JLA 8.1000 Fixed Angle Rotor

      Large-volume rotor made of anodized aluminum with carbon-fiber canisters for large-volume processing, pelleting of bacteria, cell organelles, viruses and precipitates.

    • J-LITE JLA 16.250 Fixed Angle Rotor

      J-LITE JLA 16.250 Fixed Angle Rotor

      Large-volume rotor made of anodized aluminum with carbon-fiber canisters for harvesting bacteria and cell membranes, processing tissue homogenates, and separating particulates. Comes with a biosafe** lid. This rotor is a lightweight alternative to conventional 6 x 250 rotors; empty rotor weight is 10.3 kg (22.7 lb).

      ** BioSafe is a term intended to describe the enhanced Biosafety features of our products

Ultracentrifuge Floor Model

    • NVT 65 Near-Vertical Tube Rotor

      NVT 65 Near-Vertical Tube Rotor

      A BioEnhanced*, near-vertical tube rotor made of titanium and used for the separation of plasmid or mitochondrial DNA in CsC1 gradients.

      For use in instruments classified: H R S

      Note: Non-precipitating solutions up to 1.7 g/mL in density can be run in this rotor without a reduction in rotor speed.

      *BioEnhanced is a term intended to describe our products' enhanced level of biocontainment by design.

    • NVT 65.2 Near-Vertical Tube Rotor

      NVT 65.2 Near-Vertical Tube Rotor

      A titanium near-vertical tube rotor used for the separation of plasmid, mitochondrial, or chromosomal DNA, proteoglycans, and lipoproteins.

      For use in instruments classified: H R S

      Note: Solutions up to 1.7 g/mL in density can be run in this rotor without a reduction in rotor speed.

    • NVT 90 Near-Vertical Tube Rotor

      NVT 90 Near-Vertical Tube Rotor

      A BioEnhanced*, near-vertical tube rotor made of titanium and used for the separation of plasmid or mitochondrial DNA in CsCI gradients in 2.5 – 4 hours.

      For use in instruments classified: H R S

      Note: Non-precipitating solutions up to 1.7 g/mL in density can be run in this rotor without a reduction in rotor speed. For speed reductions for CsC1 prescription, refer to rotor manual.

      *BioEnhanced is a term intended to describe our products' enhanced level of biocontainment by design.

    • NVT 100 Near-Vertical Tube Rotor

      NVT 100 Near-Vertical Tube Rotor

      A BioEnhanced*, near-vertical tube rotor made of titanium used for the separation of plasmid or mitochondrial DNA in CsCI gradients in 2.5 – 4 hours.

      For use in instruments classified: R S

      Note: Non-precipitating solutions up to 1.7 g/mL in density can be run in this rotor without a reduction in rotor speed. For speed reductions for CsCl prescription, refer to rotor manual.

      *BioEnhanced is a term intended to describe our products' enhanced level of biocontainment by design.

    • SW 28 Swinging-Bucket Rotor

      For use in instruments classified: H R S

      This BioEnhanced* rotor has an aluminum head and titanium buckets. It develop centrifugal forces for the separation of subcellular particles and viruses in density gradients.

      *BioEnhanced is a term intended to describe our products' enhanced level of biocontainment by design.

    • SW 28.1 Swinging-Bucket Rotor

      This BioEnhanced* rotor has an aluminum head and titanium buckets. It develop centrifugal forces for the separation of subcellular particles and viruses in density gradients.

      *BioEnhanced is a term intended to describe our products' enhanced level of biocontainment by design.

    • SW 32 Ti Swinging-Bucket Rotor

      SW 32 Ti Swinging-Bucket Rotor

      This BioEnhanced*, titanium rotor develops centrifugal forces for the separation of subcellular particles and viruses in density gradients.

      *BioEnhanced is a term intended to describe our products' enhanced level of biocontainment by design.

    • SW 40 Ti Swinging-Bucket Rotor

      This BioEnhanced* rotor has a titanium head and red buckets. The rotor develops centrifugal forces for the separation and purification of small particles. Typical applications include separation of RNA, proteins, and subcellular particles in solution using rate zonal centrifugation.

      For use in instruments classified: H R S

      *BioEnhanced is a term intended to describe our products' enhanced level of biocontainment by design.

    • SW 41 Ti Swinging-Bucket Rotor

      SW 41 Ti Swinging-Bucket Rotor

      For use in instruments classified: H R S

      A BioEnhanced* rotor with a titanium head and black buckets. Typical applications include separation of RNA, proteins, and subcellular particles in solution using rate zonal centrifugation. Approximate sample volume per tube is 0.5 mL, with a gradient volume of about 12.5 mL.

      *BioEnhanced is a term intended to describe our products' enhanced level of biocontainment by design.

    • SW 55 Ti Swinging-Bucket Rotor

      BioEnhanced*, Swinging-Bucket Rotor, Titanium Head, and Buckets (red buckets)

      For use in instruments classified: H R S

      Typical applications for the SW 55 Ti rotor include separation of DNA, RNA, proteins, and subcellular particles in density gradients, and banding RNA- containing viruses in sedimentation equilibrium studies. Approximate sample volume per tube is 0.2 mL, with a gradient volume of about 4.8 mL.

      *BioEnhanced is a term intended to describe our products' enhanced level of biocontainment by design.

    • SW 60 Ti Swinging-Bucket Rotor

      Titanium Head, and Buckets

      For use in instruments classified: H R S

      This BioEnhanced* rotor develops centrifugal forces for the separation and purification of small particles. Typical applications include separation of RNA, proteins, and subcellular particles in solution using rate zonal centrifugation.

      *BioEnhanced is a term intended to describe our products' enhanced level of biocontainment by design.

    • Type 19 Fixed-Angle Rotor

      Type 19 Fixed-Angle Rotor

      A BioEnhanced*, aluminum rotor for that develops centrifugal forces for processing biological materials. Up to 1500 mL of gradient and sample volume can be centrifuged per run.

      For use in instruments classified: H R S

      *BioEnhanced is a term intended to describe our products' enhanced level of biocontainment by design.

    • Type 25 Fixed-Angle Rotor

      An aluminum rotor for differential flotation of many small samples of lipoproteins. Approximately 100 mL of gradient and sample can be centrifuged per run.

      For use in instruments classified: H R S

    • Type 42.2 Ti Fixed-Angle Rotor

      Type 42.2 Ti Fixed-Angle Rotor

      A BioEnhanced*, titanium rotor that develops centrifugal forces that can efficiently separate intact viruses, microsomes, and lipoproteins in small-volume samples.

      For use in instruments classified: H R S

      *BioEnhanced is a term intended to describe our products' enhanced level of biocontainment by design.

    • Type 45 Ti Fixed-Angle Rotor

      Type 45 Ti Fixed-Angle Rotor

      A BioEnhanced*, titanium rotor that develops centrifugal forces sufficient for applications including pelleting of small particles, and purification of viruses and subcellular organelles using sucrose gradients. Up to 564 mL of sample and gradient can be centrifuged per run.

      For use in instruments classified: H R S

      Note: This rotor cannot be used in 40 000 rpm F or H instruments or the L5-50E unless the instruments have been upgraded with a diffusion pump and vacuum indicator.

      *BioEnhanced is a term intended to describe our products' enhanced level of biocontainment by design.

    • Type 50.2 Ti Fixed-Angle Rotor

      Type 50.2 Ti Fixed-Angle Rotor

      A BioEnhanced*, titanium rotor that develops centrifugal forces sufficient for the differential separation of particles, and for equilibrium sedimentation of particles such as DNA and viruses. Up to 468 mL of gradient and sample volume can be centrifuged per run.

      For use in instruments classified: H R S

      *BioEnhanced is a term intended to describe our products' enhanced level of biocontainment by design.

    • Type 50.4 Ti Fixed-Angle Rotor

      Type 50.4 Ti Fixed-Angle Rotor

      A BioEnhanced*, titanium rotor that develops centrifugal forces sufficient for the differential separation of particles, and for equilibrium sedimentation of particles such as DNA and viruses. Up to 286 mL of gradient and sample volume can be centrifuged per run.

      For use in instruments classified: H R S

      *BioEnhanced is a term intended to describe our products' enhanced level of biocontainment by design.

    • Type 70 Ti Fixed-Angle Rotor

      Type 70 Ti Fixed-Angle Rotor

      A BioEnhanced*, titanium rotor that develops centrifugal forces sufficient for applications including pelleting of small particles, and purification of viruses and subcellular organelles using sucrose gradients. Up to 312 mL of sample and gradient can be centrifuged per run.

      For use in instruments classified: H R S

      *BioEnhanced is a term intended to describe our products' enhanced level of biocontainment by design.

    • Type 70.1 Ti Fixed-Angle Rotor

      A titanium rotor that develops centrifugal forces sufficient for applications including pelleting of small particles, and purification of viruses and subcellular organelles using sucrose gradients. Up to 162 mL of sample and gradient can be centrifuged per run.

      For use in instruments classified: H R S

    • Type 90 Ti Fixed-Angle Rotor

      Type 90 Ti Fixed-Angle Rotor

      A BioEnhanced*, titanium rotor used for five-hour separation of plasmid DNA and rapid differential centrifugation of small particles.

      The rotor develops centrifugal forces sufficient for the differential separation of particles, and for equilibrium  sedimentation of particles such as DNA and viruses. Up to 108 mL of gradient and sample volume can be centrifuged per run.

      For use in instruments classified: H R S

      *BioEnhanced is a term intended to describe our products' enhanced level of biocontainment by design.

    • Type 100 Ti Fixed-Angle Rotor

      Type 100 Ti Fixed-Angle Rotor

      A BioEnhanced*, titanium rotor for rapid separation of plasmid DNA and rapid differential centrifugation of small particles.

      For use in instruments classified: R S

      *BioEnhanced is a term intended to describe our products' enhanced level of biocontainment by design.

    • VTi 50 Vertical Tube Rotor

      VTi 50 Vertical Tube Rotor

      The BioEnhanced*, titanium rotor develops centrifugal forces that can efficiently band DNA or isolate proteins on density gradients. Up to 312 mL of gradient and sample can be centrifuged per run.

      For use in instruments classified: H R S

      Note: Solutions up to 1.7 g/mL in density can be run in this rotor without reduction in rotor speed.

      Major applications: Isopycnic and rate-zonal centrifugation.

      *BioEnhanced is a term intended to describe our products' enhanced level of biocontainment by design.

    • VTi 65.1 Vertical Tube Rotor

      VTi 65.1 Vertical Tube Rotor

      For use in instruments classified: H R S

      This titanium rotor is particularly appropriate for the separation of closed circular plasmid DNA from linear DNA in cesium chloride-ethidium bromide gradients. RNA will pellet; protein and other cellular components with low buoyant density in CsCl will float. The supercoiled plasmid will band in the central area of the tube with a starting CsCl density of approximately 1.55 g/mL. With typical sample loading, the plasmid band will not be in contact with the pellet or the components of the sample which float.

      Note: Solutions up to 1.7 g/mL in density can be run in this rotor without reduction in rotor speed.

    • VTi 65.2 Vertical Tube Rotor

      VTi 65.2 Vertical Tube Rotor

      A BioEnhanced*, vertical-tube rotor made of titanium and used for isopycnic and rate zonal centrifugation of small particles.

      For use in instruments classified: H R S

      Note: Solutions up to 1.7 g/mL in density can be run in this rotor without reduction in rotor speed.

      *BioEnhanced is a term intended to describe our products' enhanced level of biocontainment by design.

    • VTi 90 Vertical Tube Rotor

      VTi 90 Vertical Tube Rotor

      A BioEnhanced*, vertical-tube rotor made of titanium and used for high-speed density gradient separations.

      For use in instruments classified: H R S

      Note: Solutions up to 1.7 g/mL in density can be run in this rotor without reduction in rotor speed.

      *BioEnhanced is a term intended to describe our products' enhanced level of biocontainment by design.

Ultracentrifuge Tabletop

    • MLA-50 Fixed-Angle Rotor

      MLA-50 Fixed-Angle Rotor

      This BioEnhanced*, aluminum rotor is used for large volume separations of subcellular particles, viruses, lipoproteins, RNA, and plasmid DNA.

      * BioEnhanced is a term intended to describe the enhanced BioSafety of our products.

    • MLA-55 Fixed-Angle Rotor

      MLA-55 Fixed-Angle Rotor

      This BioEnhanced*, Aluminum rotor is commonly used for plasmid DNA separations.

      *BioEnhanced is a term intended to describe the enhanced BioSafety of our products.

    • MLA-80 Fixed-Angle Rotor

      MLA-80 Fixed-Angle Rotor

      A titanium rotor used for large volume differential sedimentation (pelleting) of subcellular organelles and viruses.

    • MLA-130 Fixed-Angle Rotor

      MLA-130 Fixed-Angle Rotor

      A titanium rotor useful for rapid differential sedimentation (pelleting) of small particles such as subcellular organelles and viruses.

    • MLA-150 Fixed-Angle Rotor

      MLA-150 Fixed-Angle Rotor

      A BioEnhanced*, titanium rotor useful for separation of subcellular particles (pelleting, rate-zonal gradient), viruses (pelleting, rate-zonal gradient), protein purification/isolation (pelleting, rate-zonal gradient), DNA (pelleting, CsCl isopycnic), RNA (CsCl pelleting, density gradient), Lipoproteins (differential flotation or gradient).

      *BioEnhanced is a term intended to describe the enhanced BioSafety of our products.

    • MLN-80 Near-Vertical Tube Rotor

      MLN-80 Near-Vertical Tube Rotor

      This BioEnhanced*, titanium rotor is used for rapid contamination-free isopycnic isolation of plasmid DNA.

      *BioEnhanced is a term intended to describe the enhanced BioSafety of our products.

    • MLS-50 Swinging-Bucket Rotor

      MLS-50 Swinging-Bucket Rotor

      This BioEnhanced*, aluminum rotor is used for rate-zonal separations of subcellular organelles, pelleting and isopycnic separations of RNA.

      *BioEnhanced is a term intended to describe the enhanced BioSafety of our products.

    • TLA-55 Fixed-Angle Rotor

      TLA-55 Fixed-Angle Rotor

      This BioCertified, aluminum rotor is used for the rapid pelleting of nucleic acid precipitates in 1.5 mL tubes.

      BioCertified is a term used to describe our products which have been tested and validated to demonstrate containment of microbiological aerosols by an independent, third-party facility (Health Protection Agency, Porton Down, UK or USAMRIID, Ft. Detrick, MD, USA). Improper use or maintenance may affect seal integrity and, thus, containment

    • TLA-100 Fixed-Angle Rotor

      TLA-100 Fixed-Angle Rotor

      This titanium rotor is ideal for pelleting of subcellular fractions; proteins; RNA.

      Note: Non-precipitating solutions up to 1.7 g/mL in density can be run in this rotor without a reduction in rotor speed.

    • TLA-100.3 Fixed-Angle Rotor

      TLA-100.3 Fixed-Angle Rotor

      This BioEnhanced*, titanium rotor is used for the pelleting of subcellular fractions in 5-30 minutes; pelleting RNA in 1-2 hours; plasmid DNA separation in 6 hours.

      Note: Non-precipitating solutions up to 1.7 g/mL in density can be run in this rotor without a reduction in rotor speed.

      *BioEnhanced is a term intended to describe the enhanced BioSafety of our products.

    • TLA-110 Fixed-Angle Rotor

      A BioCertified, titanium rotor useful for moderate volume differential sedimentation (pelleting) of subcellular organelles and viruses.

      BioCertified is a term used to describe our products which have been tested and validated to demonstrate containment of microbiological aerosols by an independent, third-party facility (Health Protection Agency, Porton Down, UK or USAMRIID, Ft. Detrick, MD, USA). Improper use or maintenance may affect seal integrity and, thus, containment

    • TLA-120.1 Fixed-Angle Rotor

      TLA-120.1 Fixed-Angle Rotor

      This BioEnhanced*, Titanium rotor is ideal for the pelleting of subcellular fractions in 5-30 minutes; plasmid DNA separation in 3 hours.

      *BioEnhanced is a term intended to describe the enhanced BioSafety of our products.

    • TLA-120.2 Fixed-Angle Rotor

      TLA-120.2 Fixed-Angle Rotor

      This BioCertified, Titanium rotor is used for pelleting of subcellular fractions in 5-30 minutes, plasmid DNA separation in 3 hours.

      BioCertified is a term used to describe our products which have been tested and validated to demonstrate containment of microbiological aerosols by an independent, third-party facility (Health Protection Agency, Porton Down, UK or USAMRIID, Ft. Detrick, MD, USA). Improper use or maintenance may affect seal integrity and, thus, containment

    • TLN-100 Near-Vertical Tube Rotor

      TLN-100 Near-Vertical Tube Rotor

      This BioEnhanced*, titanium rotor is used for Plasmid DNA‑Separations in 4 hours.

      Note: Non-precipitating solutions up to 1.7 g/mL in density can be run in this rotor without a reduction in rotor speed.

      * BioEnhanced is a term intended to describe the enhanced BioSafety of our products.

    • TLN-120 Near-Vertical Tube Rotor

      TLN-120 Near-Vertical Tube Rotor

      A BioEnhanced*, titanium rotor used for Plasmid DNA Separations in 11/2 hours.**

      *BioEnhanced is a term intended to describe the enhanced BioSafety of our products.

      **To accommodate the TLN-120 Rotor, the TL-100 ultracentrifuge must be updated with a new drive spindle and operating software (modification kit number 360477).

      **Using Optima MAX-XP, Optima MAX-TL or Optima TLX.

      Note: Non-precipitating solutions up to 1.7 g/mL in density can be run in this rotor without a reduction in rotor speed.

    • TLS-55 Swinging-Bucket Rotor

      TLS-55 Swinging-Bucket Rotor

      This BioCertified, titanium rotor is used for RNA pelleting in 2-3 hours; subcellular fractionation in sucrose gradients, protein separations in sucrose gradients.

      Note: Non-precipitating solutions up to 1.7 g/mL in density can be run in this rotor without a reduction in rotor speed.

      BioCertified is a term used to describe our products which have been tested and validated to demonstrate containment of microbiological aerosols by an independent, third-party facility (Health Protection Agency, Porton Down, UK or USAMRIID, Ft. Detrick, MD, USA).   Improper use or maintenance may affect seal integrity and, thus, containment

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