The Optima AUC combines the power of a centrifuge to provide particle sedimentation with the functionality of optical modules to detect the sedimentation over time. It provides the data you need even faster, so you can make new discoveries in less time.
Summary of features and benefits:
- Enables sample recovery
- Avoids dilution effects
- Analyzes samples in a matrix-free environment
- Has minimal buffer constraints
- Enables detection at low concentrations
- Requires low sample volume (0.1 mL minimum)
- Delivers high-throughput analysis – up to 7 samples in a single run
- Requires no standards – relies upon first principles of thermodynamics
- Analyzes a wide array of particles in native, matrix-free conditions, including:
- Drug conjugates
- Viral payload
- Provides data which can answer more critical questions than any other comparable technique, including:
- Enables precise analysis of complex systems at discrete wavelengths (up to 20) in minimal time.
- Generates faster results as acquisition time is no longer additive.
- Provides even more scans with the use of an An-50 Ti 8-hole rotor.
Ease of Use
- Modular design provides absorbance and interference optical systems—with the option to install up to 3 independent, simultaneous detection systems (see photo).
- User-friendly touchscreen display indicates intuitive progression of experimental design.
- Remote monitoring capabilities let you set up, monitor and extract data from virtually any location.
- Compatibility with ProteomeLab XL cells and rotors—and widely used analytical software (e.g., UltraScan, SEDFIT/SEDPHAT, SEDANAL, DCDT+)—helps simplify the upgrade process.
- Delivers cleaner data by providing higher radial resolution and higher signal-to-noise ratio.
- Delivers higher precision, reproducibility and accuracy than the ProteomeLab XL.
Optima AUC Specifications
The Optima AUC is manufactured in the USA in an ISO 13485-registered facility and designed/tested to meet standards and regulations of CE mark and CSA when used as a complete system of ultracentrifuge, rotors and labware.
||1,000 − 60,000 RPM in 100 RPM increments
||289,848 x g
|Ambient temperature range
||10° - 30°C
|Noise level (1 m in front of instrument)
||< 60 dBA
||< 5 µm (0.7 Pa)
|Max heat output under steady-state conditions
||3,400 Btu/hr (1.0 kW)
For remote monitoring: GUI (any OS)
||94 cm (37") wide
68.1 cm (26.8") deep
125.7 cm (49.5 in)
||508.5 kg (1121 lb)
|Data acquisition rate
||ABS: < 7 seconds/sectori
INT: < 5 seconds/scan
|Maximum # of wavelengths
||20 (190 nm - 800 nm)
||± 0.5 nm
|Lowest radial resolution
|ABS flash lamp frequency
|ABS dynamic range
|| 0.1 O.D. < c < 1.5 O.D.
|CCD camera specifications
||2048 x 1088 pixels
||≥ 10 fringes/cell
|Usable concentration ranges
||ABS: 0.005 - ~ 1-2 mg/mL LH
INT: 0.25 - ~ 4-5 mg/mL BSA
|Maximum unique users/passwords
||50 (web interface)
|Maximum user‐defined programs
||800 GB of space for methods to be stored. There are 10,000 number assignments available
For the first time, these advanced features enable revolutionary new multi-wavelength experiments, an entirely new class of experimental designs that can exploit the presence of multiple chromophores in complex mixtures through spectral decomposition.
The University of Texas Health Science Center
At optimal rotor speeds and 10 µm radial resolution