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Monitoring cellular immune responses is a prerequisite for the development of cancer vaccines. Key objectives of immune monitoring include determining the efficacy of a vaccine to induce or augment a specific T cell response as well as the role that regulatory T cells (Tregs) - a small subset of supressor cells - have on the development of T cell responses.
We offer a variety of tools and high-quality immune monitoring reagents and life sciences technologies designed to help researchers enhance their understanding of the immune system, from B and T cells to molecular pathways.
T cells are a diverse population of lymphocytes that work to structure appropriate cell-mediated immune responses. T cells are developed in the bone marrow and matured in the thymus where they undergo strict selection processes to confirm their recognition of foreign antigens. All T cells express either the alpha-beta heterodimeric T cell receptor (TCR) or the gamma-delta heterodimeric TCR. The TCR assigns antigen specificity and is essential for the role the T cell must play during the adaptive immune response.
The role of T cells must is broken down based on the phenotype and function of T cell subsets. There are several subsets each with a distinct function including the T helper, T cytolytic, T regulatory or gamma/delta T cells.
T regulatory cells are a subset of CD4 lymphocytes that are critical to maintain homeostasis. TREGS can affect immune cell homeostasis via several mechanisms including the production of cytokines and other secreted proteins, direct cell-mediated regulation and cytolytic activities.
B cells mediate the creation of antibodies to bind specifically to foreign antigens including bacteria and microbial toxins. B cells, along with T cells, respond to harmful pathogens and establish immunological memory within an organism. These cells are characterized by their expression of clonally diverse cell surface markers (“cluster of differentiation” or CD marker) and secreted immunoglobulin B cell receptor (BCR) molecules.
B-cell development is initiated in the fetal liver and in the bone marrow in adults; however, subsequent functional maturation occurs in secondary lymphoid tissue. Early in development, the pre-B cell receptor (pre-BCR) plays a pivotal role in assuring the continued maturation and survival of B cell progenitors.
Based on the ONE Study panels, expert flow cytometry labs have designed and optimized the marker and dye selections in DuraClone IM panels for use in clinical research studies.
Single-unit, dry-format reagent cocktails help minimize variability for studies carried out across multiple sites. DuraClone IM panels, each provided with specific compensation kits, standardize high-content flow for streamlined use in clinical research.
DuraClone IM Phenotyping Basic tube delineates principal lymphocyte subsets as well as subpopulations of monocytes.
DuraClone IM B Cell tube allows for identification of late maturation stages of B cells, such as: transitional stage, Isotype class switch, naive and memory stages.
DuraClone IM T Cell Subsets tube indicates maturation stages of T cells, covering naive, effector, memory and terminal differentiation stages.
DuraClone IM Dendritic Cell tube discriminates leukocyte-lineage positive cells and identifies plasmacytoid dendritic cells and 3 major subsets of myeloid dendritic cells.
DuraClone IM TCRs tube allows for assessment of TCR αβ and γδ protein chain expression and to detect Vδ1+ and Vδ2+ subsets among TCRγδ+ T cells.
DuraClone IM Treg tube enables identification and characterization of FoxP3+ regulatory T cells using a fast permeabilization protocol.
DuraClone IM Granulocytes tube identifies major granulocyte subpopulations including assessment of CD11b, CD16, CD62L and PD-1L (CD274) co-expression.
With advanced intracellular staining chemistry for superior reproducibility, PerFix-nc is designed to detect surface and intracellular antigens simultaneously with fewer or no wash steps. Now available for in vitro diagnostic use in the United States and Canada, PerFix-nc carries both CE-IVD and IVD labeling, ensuring complete regulatory compliance.
The PerFix-nc kit offers the fastest commercially available procedure for simultaneous detection of surface and intracellular antigens. Use PerFix-nc to lyse red blood cells and induce permeability in the cytoplasmic membrane of leukocytes for intra- and extracellular staining for flow cytometry analysis.
We offer several hundred IVD and CE-IVD single-color reagents, multi-color cocktails, kits, assays and software to fit the needs of clinical customers from sample preparation to data analysis.
*Total time. Less than 15-minutes hands-on time.
Example of Kappa/Lambda staining
Low Fix Wash PerFix-nc procedure on whole blood sample. Staining with Lambda-PE and Kappa-FITC.
Example of Intracellular TdT staining on normal and pathological blood samples
Low Fix No Wash PerFix-nc procedure on normal and pathological blood samples. Staining with TdT-FITC.
Aprajita Priyadarshini, Sr. Applications Scientist, Beckman Coulter
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DuraClone is an innovative approach for dry reagents for flow cytometry. The proprietary process results in a product that is consistent from tube to tube and lot to lot and eliminates the need for cold chain storage. The process is distinct...
John Tigges Technical Director, Flow Cytometry Core Beth Israel Deaconess Medical Center, Boston, MA
Stefano Pluchino, MD, PhD Wellcome Trust-Medical Research Council Stem Cell Institute Clifford Allbutt Building - Cambridge Biosciences Campus Department of Clinical Neurosciences
William Godfrey, Ph.D. Manager, Reagent & Application Development, Beckman Coulter. Inc
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7-AAD Viability Dye
IVD & CE
APC-Alexa Fluor® 700
APC-Alexa Fluor® 750
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