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Applications

Transform the efficiency and reliability of your research with Beckman Coulter automated lab protocols. Discover protocol information and insights relevant to your application here.

Cell Signaling

Cell signaling is part of a complex communication system that governs basic cellular activities and coordinates cell actions including divison, differentiation, metabolism and death. Research indicates errors in intracellular processing are responsible for diseases such as cancer, autoimmunity and diabetes.
 
The initiation of cell signaling pathways typically occurs when a cellular receptor binds cognate ligand. Subsequent pathway activation depends on the type of receptor activated by ligand binding. In some cases, ligand binding itself can result in transformational changes in the receptor whereas protein-to-protein interactions are induced as a result of a cellular receptor binding its cognate ligand. These protein-protein interactions are phosphorylation/dephosphorylation events that cascade associated signaling molecules through kinases and phosphatases. Themitogen-activated protein kinase pathways (MAPK) is one example that is known to alter cellular proliferation as a result of the downstream phosphorylation of signaling molecules.

PerFix-nc

Stain in a single step with PerFix-nc

With advanced intracellular staining chemistry that delivers excellent reproducibility PerFix-nc efficiently detects surface and intracellular antigens simultaneously with fewer or no wash steps.

Now available for in vitro diagnostic use in the United States and Canada, PerFix-nc carries both CE-IVD and IVD labeling, ensuring complete regulatory compliance.

 

Precise results in 45 minutes-or-less*

The PerFix-nc kit offers the fastest commercially available procedure for simultaneous detection of surface and intracellular antigens. Use PerFix-nc to lyse red blood cells and induce permeability in the cytoplasmic membrane of leukocytes for intra and extracellular staining in flow cytometry
analysis.

We offer several hundred IVD and CE-IVD single-color reagents, multi-color cocktails, kits, assays and software to fit the needs of clinical customers from sample preparation to data analysis.

*Total time. Less than 15-minutes hands-on time.
intracellular staining


Simplified workload

  • Intracellular staining as simple as surface staining
  • No-centrifuge, no-wash procedure
  • Room temperature (no water bath or ice required)
  • Automation-ready
  • One kit, flexible protocols, multiple applications

 

Proven performance

  • No wash, less preparation for better reproducibility
  • Compatible with all Beckman Coulter dyes
  • Retains versatility with optional wash
  • Adapts to specific antigen detection requirements
  • Remarkable cell recovery and gate purity
  • Enhances signal-to-noise ratio for intracellular staining
Intracellular staining

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Adapters - Clinical Diagnostics

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Adapters

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Centrifuges - Clinical Diagnostics

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Centrifuges

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Flow Cytometry Instruments & Software - Clinical Diagnostics

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Lasers
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Flow Cytometry Instruments & Software - Research and Discovery

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Nucleic Acid - Clinical Diagnostics

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Nucleic Acid Sample Preparation

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Liquid Handling Accessories - Clinical Diagnostics

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Liquid Handling Accessories

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Liquid Handling Instruments - Clinical Diagnostics

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Liquid Handlers

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Liquid Handling Plates - Clinical Diagnostics

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Liquid Handling Plates

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Liquid Handling Reservoirs - Clinical Diagnostics

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Liquid Handling Reservoirs

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Liquid Handling Tips - Clinical Diagnostics

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Liquid Handling Tips

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Particle Counter/Cell Sizing and Processing

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Flow Cytometry Reagents - Clinical Diagnostics

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IM3454U

7.1 (NG2 antigen)

Human

PE

7.1

ASR

$490.00

A07704

7-AAD Viability Dye

IVD & CE

$205.00

IM1360U

CD117 (c-kit)

Human

PE

95C3

ASR

$475.00

IM2657U

CD117 (c-kit)

Human

PC5

95C3

ASR

$540.00

IM3638

CD117 (c-kit)

Human

APC

104D2D1

ASR

$490.00

IM3698

CD117 (c-kit)

Human

PC7

104D2D1

ASR

$620.00

IM2732U

CD117 (c-kit)

Human

PE

104D2D1

ASR

$475.00

IM2733U

CD117 (c-kit)

Human

PC5

104D2D1

ASR

$540.00

A66333

CD117 (c-kit)

Human

PC5.5

104D2D1

ASR

$580.00

A86051

CD117 (c-kit)

Human

APC-Alexa Fluor® 750

104D2D1

ASR

$580.00

A53365

CD122 (IL-2R beta)

Human

PC7

CF1

ASR

$620.00

A32535

CD123 (IL-3Ralpha)

Human

PE

SSDCLY107D2

ASR

$425.00

B06376

CD123 (IL-3Ralpha)

Human

APC

SSDCLY107D2

ASR

$540.00

B13647

CD123 (IL-3Ralpha)

Human

PC7

SSDCLY107D2

ASR

$550.00

B20022

CD123 (IL-3Ralpha)

Human

PC5.5

SSDCLY107D2

ASR

$530.00

B20027

CD123 (IL-3Ralpha)

Human

ECD

SSDCLY107D2

ASR

$530.00

B14808

CD123 (IL-3Ralpha)

Human

PE

9F5

ASR

$460.00

B24028

CD123 (IL-3Ralpha)

Human

APC-Alexa Fluor® 700

SSDCLY107D2

ASR

$530.00

IM1979

CD126 (IL-6Ralpha)

Human

PE

M91

ASR

$455.00

IM1980U

CD127 (IL-7Ralpha)

Human

PE

R34.34

ASR

$490.00

A64617

CD127 (IL-7Ralpha)

Human

PC5

R34.34

ASR

$800.00

A64618

CD127 (IL-7Ralpha)

Human

PC7

R34.34

ASR

$650.00

A71116

CD127 (IL-7Ralpha)

Human

APC-Alexa Fluor® 700

R34.34

ASR

$580.00

B12700

CD127 (IL-7Ralpha)

Human

APC-Alexa Fluor® 750

R34.34

ASR

$540.00

B25366

CD127 (IL-7Ralpha)

Human

FITC

R34.34

ASR

$415.00

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Flow Cytometry Reagents - Research and Discovery

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IM1977

CD116 (GM-CSFR)

Human

PE

SCO6

RUO

$455.00

A22360

CD120b (TNF-R2)

Human

PE

80M2

RUO

$540.00

IM1846

CD158b1/b2,j

Human

Purified

GL183

RUO

$265.00

IM2748

CD158e1/e2

Human

Purified

Z27.3.7

RUO

$340.00

IM1607

CD244 (2B4)

Human

Purified

C1.7

RUO

$340.00

6607112

CD25

Human

ECD

B1.49.9

RUO

$540.00

A49080

CD278 (ICOS)

Human

PE

ISA-3

RUO

$455.00

6607108

CD28

Human

PC5

CD28.2

RUO

$510.00

6607111

CD28

Human

ECD

CD28.2

RUO

$540.00

IM2409

CD31

Human

PE

1F11

RUO

$455.00

A44226

CD85a (ILT5)

Human

PE

7H5

RUO

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IM0117

CD9

Human

Purified

ALB6

RUO

$265.00

IM1506

CD95

Human

FITC

UB2

RUO

$395.00

IM1739

CD95

Human

PE

UB2

RUO

$455.00

B26976

PerFix EXPOSE, 75 test, RUO

RUO

$400.00

A88928

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204)

Human

Alexa Fluor® 488

E10

RUO

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A88924

Phospho-Stat3 (Tyr705) XP™

Human

Alexa Fluor® 488

D3A7

RUO

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A88883

Zap-70

Human

Alexa Fluor® 647

136F12

RUO

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Showing 1 to 18 of 18 Results

Rotors - Clinical Diagnostics

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Rotors

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Tubes & Bottles - Clinical Diagnostics

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Tubes & Bottles

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